干旱区生态环境知识资源中心(Arid): Statistical methodology for the evaluation of leukocyte data in wild reptile populations: A case study with the common wall lizard (Podarcis muralis)

Arid

DOI	10.1371/journal.pone.0237992
	Statistical methodology for the evaluation of leukocyte data in wild reptile populations: A case study with the common wall lizard (Podarcis muralis)
	Sacchi, Roberto; Mangiacotti, Marco; Scali, Stefano; Coladonato, Alan J.; Pitoni, Silvia; Falaschi, Mattia; Zuffi, Marco A. L.
通讯作者	Sacchi, R
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2020
卷号	15 期号:8
英文摘要	The leukocyte profile has the potential to be a reliable method to measure health conditions and stress in wild animals, but limitations occur because current knowledge on reference intervals is largely incomplete, especially because data come from studies on captive animals involving few individuals from single populations. Here we propose a general framework for achieving reliable leukocyte reference intervals, encompassing a set of internal and external factors, potentially affecting the leukogram. To do so, we present a systematic survey of the hematology of the common wall lizard,Podarcis muralis, involving 794 lizards from 54 populations over the whole geographic range of the species in Italy. Reference intervals for white blood cell (WBC) and leukocyte differential count were obtained by using linear mixed models in a Bayesian framework. The application of the procedure clearly showed that both internal (sex and size) and external (latitude and season) factors are a source of variation of leukocyte profile. Furthermore, the leukogram of common wall lizard has a strong variability among populations, which accounts for more than 50% of the whole variation. Consequently, some common assumptions used in studies on captive individuals are no longer supported in wild populations, namely, i) any group of individuals is a representative sample, ii) any population is representative of all others, iii) geographic clines do not occur over the species range, and iv) seasonal variation has limited effects. We encourage researchers aimed at the definition of leukocyte reference intervals for wild populations of reptiles to involve a large number of populations over a wide geographic range inad hocstatistical models to disentangle local and geographic effects on leukocyte profile variation.
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000566205500021
WOS关键词	FREE-RANGING DESERT ; REFERENCE INTERVALS ; BLOOD-CELLS ; ACTIVITY PATTERNS ; REFERENCE VALUES ; HEMATOLOGY ; BIOCHEMISTRY ; MORPHOLOGY ; TORTOISES ; STRESS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326041
作者单位	[Sacchi, Roberto; Mangiacotti, Marco; Coladonato, Alan J.; Pitoni, Silvia] Univ Pavia, Dipartimento Sci Terra & Ambiente, Pavia, Italy; [Mangiacotti, Marco; Scali, Stefano; Falaschi, Mattia] Museo Storia Nat Milano, Milan, Italy; [Falaschi, Mattia] Univ Milan, Dipartimento Sci & Polit Ambientali, Milan, Italy; [Zuffi, Marco A. L.] Univ Pisa, Museo Storia Nat, Calci, Italy
推荐引用方式 GB/T 7714	Sacchi, Roberto,Mangiacotti, Marco,Scali, Stefano,et al. Statistical methodology for the evaluation of leukocyte data in wild reptile populations: A case study with the common wall lizard (Podarcis muralis)[J],2020,15(8).
APA	Sacchi, Roberto.,Mangiacotti, Marco.,Scali, Stefano.,Coladonato, Alan J..,Pitoni, Silvia.,...&Zuffi, Marco A. L..(2020).Statistical methodology for the evaluation of leukocyte data in wild reptile populations: A case study with the common wall lizard (Podarcis muralis).PLOS ONE,15(8).
MLA	Sacchi, Roberto,et al."Statistical methodology for the evaluation of leukocyte data in wild reptile populations: A case study with the common wall lizard (Podarcis muralis)".PLOS ONE 15.8(2020).