干旱区生态环境知识资源中心(Arid): Keratinophilic fungi: Specialized fungal communities in a desert ecosystem identified using cultured-based and Illumina sequencing approaches

Arid

DOI	10.1016/j.micres.2020.126530
	Keratinophilic fungi: Specialized fungal communities in a desert ecosystem identified using cultured-based and Illumina sequencing approaches
	Hamm, Paris S.; Mueller, Rebecca C.; Kuske, Cheryl R.; Porras-Alfaro, Andrea
通讯作者	Porras-Alfaro, A
来源期刊	MICROBIOLOGICAL RESEARCH
ISSN	0944-5013
EISSN	1618-0623
出版年	2020
卷号	239
英文摘要	Soil fungi in desert ecosystems are adapted to harsh environmental conditions such as high soil surface temperatures and limited organic matter and water. Given limited carbon inputs from plant material, heterotrophic fungi likely use unconventional sources of carbon in these systems. A baiting method was used to culture keratinophilic fungi from biocrust and rhizosphere soils in an arid grassland in Utah, USA. Fungi were baited using llama and sheep wool, horsehair, and snakeskin on two media, and pure cultures were identified using ITS and LSU rRNA sequences. One hundred-eighteen fungal colonies were grown, representing a total of 32 Operational Taxonomic Units (OTUs) at 97 % similarity. Cultures were dominated by the phylum Ascomycota (88 %) followed by Mucoromycota (8.6 %) and Basidiomycota (3.4 %). The orders Pleosporales, Eurotiales, Hypocreales, and Sordariales were commonly isolated, with the dominant taxa Alternaria (27 %), Aspergillus (22 %), Fusarium (11 %), and Chaetomium (8%). Thirty percent of the fungi isolated have the capacity to degrade keratin in vitro using a keratin azure assay, with Penicillium showing the highest degradation followed by Geomyces, Alternaria, and Fusarium. Although keratin degraders can be infectious, dermatophytes associated with skin infections were not isolated in culture or detected in Illumina sequencing. Illumina sequencing was used to determine general patterns in seasonal variation and habitat preference of keratinophiles. Alternaria was the most abundant genus with > 70 % of the sequences. The combination of Illumina data with culture-dependent approaches facilitated the characterization of a specialized community and confirmed the low abundance of dermatophytes in this arid site.
英文关键词	Alternaria Fungal baiting Keratin Fungal cultures Seasonal variation
类型	Article
语种	英语
开放获取类型	Bronze, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000565563300009
WOS关键词	BIOLOGICAL SOIL CRUSTS ; ALTERNARIA-ALTERNATA ; DIVERSITY ; DEGRADATION ; ENDOPHYTES ; SPORES ; ASTHMA ; MICROFUNGI ; TRANSPORT ; BACTERIAL
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326003
作者单位	[Hamm, Paris S.; Porras-Alfaro, Andrea] Western Illinois Univ, Biol Sci, Macomb, IL 61455 USA; [Hamm, Paris S.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA; [Mueller, Rebecca C.; Kuske, Cheryl R.] Los Alamos Natl Lab, Los Alamos, NM 87545 USA; [Mueller, Rebecca C.] Montana State Univ, Chem & Biol Engn, Los Alamos Natl Lab, Bozeman, MT 59717 USA
推荐引用方式 GB/T 7714	Hamm, Paris S.,Mueller, Rebecca C.,Kuske, Cheryl R.,et al. Keratinophilic fungi: Specialized fungal communities in a desert ecosystem identified using cultured-based and Illumina sequencing approaches[J],2020,239.
APA	Hamm, Paris S.,Mueller, Rebecca C.,Kuske, Cheryl R.,&Porras-Alfaro, Andrea.(2020).Keratinophilic fungi: Specialized fungal communities in a desert ecosystem identified using cultured-based and Illumina sequencing approaches.MICROBIOLOGICAL RESEARCH,239.
MLA	Hamm, Paris S.,et al."Keratinophilic fungi: Specialized fungal communities in a desert ecosystem identified using cultured-based and Illumina sequencing approaches".MICROBIOLOGICAL RESEARCH 239(2020).