干旱区生态环境知识资源中心(Arid): Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactyliferaL.) cultivar Khalas

Arid

DOI	10.1186/s40659-020-00305-3
	Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactyliferaL.) cultivar Khalas
	Al-Khateeb, Suliman A.; Al-Khateeb, Abdullatif A.; Sattar, Muhammad N.; Mohmand, Akbar S.
通讯作者	Al-Khateeb, SA
来源期刊	BIOLOGICAL RESEARCH
ISSN	0716-9760
EISSN	0717-6287
出版年	2020
卷号	53 期号:1
英文摘要	Background Soil salinity causes huge economic losses to agriculture productivity in arid and semiarid areas worldwide. The affected plants face disturbances in osmotic adjustment, nutrient transport, ionic toxicity and reduced photosynthesis. Conventional breeding approaches produce little success in combating various stresses in plants. However, non-conventional approaches, such as in vitro tissue culturing, produce genetic variability in the development of salt-tolerant plants, particularly in woody trees. Results Embryogenic callus cultures of the date palm cultivar Khalas were subjected to various salt levels ranging from 0 to 300 mM in eight subcultures. The regenerants obtained from the salt-treated cultures were regenerated and evaluated using the same concentration of NaCl with which the calli were treated. All the salt-adapted (SA) regenerants showed improved growth characteristics, physiological performance, ion concentrations and K+/Na(+)ratios than the salt non-adapted (SNA) regenerants and the control. Regression between the leaf Na(+)concentration and net photosynthesis revealed an inverse nonlinear correlation in the SNA regenerants. Leaf K(+)contents and stomatal conductance showed a strong linear relationship in SA regenerants compared with the inverse linear correlation, and a very poor coefficient of determination in SNA regenerants. The genetic fidelity of the selected SA regenerants was also tested using 36 random amplified polymorphic DNA (RAPD) primers, of which 26 produced scorable bands. The primers generated 1-10 bands, with an average of 5.4 bands per RAPD primer; there was no variation between SA regenerants and the negative control. Conclusion This is the first report of the variants generated from salt-stressed cultures and their potential adaptation to salinity in date palm cv. Khalas. The massive production of salt stress-adapted date palm plants may be much easier using the salt adaptation approach. Such plants can perform better during exposure to salt stress compared to the non-treated date palm plants.
英文关键词	Date palm Tissue culture Salt stress Ion relation K+ Na+ Photosynthesis Regenerants
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000563942200001
WOS关键词	SALINITY TOLERANCE ; GENETIC STABILITY ; CALLUS INDUCTION ; MATURE EMBRYOS ; PHOTOSYNTHESIS ; STRESS ; GROWTH ; PLANTS ; RAPD ; NA+
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325892
作者单位	[Al-Khateeb, Suliman A.] King Faisal Univ, Coll Agr & Food Sci, Dept Environm Nat Resources, POB 400, Al Hasa 31982, Saudi Arabia; [Al-Khateeb, Abdullatif A.] King Faisal Univ, Coll Agr & Food Sci, Dept Agr Biotechnol, POB 400, Al Hasa 31982, Saudi Arabia; [Sattar, Muhammad N.] King Faisal Univ, Cent Labs, Box 420, Al Hasa 31982, Saudi Arabia; [Mohmand, Akbar S.] Bacha Khan Univ Charsadda, Res Innovat & Commercializat ORIC, Charsadda, Khyber Pakhtunk, Pakistan
推荐引用方式 GB/T 7714	Al-Khateeb, Suliman A.,Al-Khateeb, Abdullatif A.,Sattar, Muhammad N.,et al. Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactyliferaL.) cultivar Khalas[J],2020,53(1).
APA	Al-Khateeb, Suliman A.,Al-Khateeb, Abdullatif A.,Sattar, Muhammad N.,&Mohmand, Akbar S..(2020).Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactyliferaL.) cultivar Khalas.BIOLOGICAL RESEARCH,53(1).
MLA	Al-Khateeb, Suliman A.,et al."Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactyliferaL.) cultivar Khalas".BIOLOGICAL RESEARCH 53.1(2020).