干旱区生态环境知识资源中心(Arid): Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens

Arid

DOI	10.1016/j.ibmb.2020.103428
	Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens
	Zhou, Xiang; Ye, Yi-Zhou; Ogihara, Mari H.; Takeshima, Mika; Fujinaga, Daiki; Liu, Cheng-Wen; Zhu, Zhen; Kataoka, Hiroshi; Bao, Yan-Yuan
通讯作者	Bao, YY
来源期刊	INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN	0965-1748
EISSN	1879-0240
出版年	2020
卷号	123
英文摘要	Ecdysteroids, insect steroid hormones, play key roles in regulating insect development and reproduction. Hemipteran insects require ecdysteroids for egg production; however, ecdysteroid synthesis (ecdysteroidogenesis) details have not been elucidated. We identified all known genes encoding ecdysteroidogenic enzymes in Nilaparvata lugens and clarified their necessity during nymphal and ovarian development. We confirmed that N. lugens utilized 20-hydroxyecdysone as an active hormone. Assays using heterologous expression of enzymes in Drosophila S2 cells showed conserved functions of enzymes Neverland, CYP306A2, CYP314A1 and CYP315A1, but not CYP302A1. RNA interference and rescue analysis using 20-hydroxyecdysone demonstrated that most of the genes were necessary for nymphal development. The identified N. lugens enzymes showed conserved functions and pathways for ecdysteroidogenesis. Knockdown of ecdysteroidogenic enzyme genes in newly molted females caused failure of egg production: less vitellogenic and mature eggs in ovaries, fewer laid eggs and embryonic development deficiency of laid eggs. Considering the high expressions of ecdysteroidogenic enzyme genes in adults and ovaries, ecdysteroidogenesis in ovaries was critical for N. lugens ovarian development. Our study presents initial evidence that hemipteran insects require ecdysteroidogenesis for ovarian development.
英文关键词	Nilaparvata lugens Ecdysteroidogenic enzyme genes 20E Oocyte development RNAi
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000562740700001
WOS关键词	BROWN PLANTHOPPER ; ECDYSONE SYNTHESIS ; HALLOWEEN GENES ; DESERT LOCUST ; MEDIATED KNOCKDOWN ; EXPRESSION ; FAMILY ; PATHWAY ; SPOOK ; METAMORPHOSIS
WOS类目	Biochemistry & Molecular Biology ; Entomology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325827
作者单位	[Zhou, Xiang; Liu, Cheng-Wen; Zhu, Zhen; Bao, Yan-Yuan] Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China; [Zhou, Xiang; Liu, Cheng-Wen; Zhu, Zhen; Bao, Yan-Yuan] Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insect Pests, Hangzhou 310058, Peoples R China; [Ye, Yi-Zhou; Ogihara, Mari H.; Takeshima, Mika; Fujinaga, Daiki; Zhu, Zhen; Kataoka, Hiroshi] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Kashiwa, Chiba 2778562, Japan; [Ogihara, Mari H.] Natl Agr & Food Res Org, Inst Livestock & Grassland Sci, 2 Ikenodai, Tsukuba, Ibaraki 3050901, Japan
推荐引用方式 GB/T 7714	Zhou, Xiang,Ye, Yi-Zhou,Ogihara, Mari H.,et al. Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens[J],2020,123.
APA	Zhou, Xiang.,Ye, Yi-Zhou.,Ogihara, Mari H..,Takeshima, Mika.,Fujinaga, Daiki.,...&Bao, Yan-Yuan.(2020).Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,123.
MLA	Zhou, Xiang,et al."Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 123(2020).