干旱区生态环境知识资源中心(Arid): Genotyping of Low beta-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers

Arid

DOI	10.1590/1678-4324-2020190150
	Genotyping of Low beta-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers
	Arslan, Mehmet; Basak, Merve; Aksu, Elcin; Uzun, Bulent; Yol, Engin
通讯作者	Arslan, M
来源期刊	BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
ISSN	1516-8913
EISSN	1678-4324
出版年	2020
卷号	63
英文摘要	Grass pea (Lathyrus sativus L.) is an important protein source in arid regions as both human and animal food. Despite its significance, the use of grass pea is limited by the presence of beta-N-oxalyl-L-a,b-diaminopropionic acid (beta-ODAP) which can cause neurological disorders. Breeding studies in grass pea have therefore focused on developing high-yielding varieties with low beta-ODAP content. However, the narrow range of genetic diversity and the restricted genomic tools in grass pea have slowed progress in such breeding. The present investigation was conducted to explore the genetic diversity of low beta-ODAP germplasm consisting of 22 accessions with 31 EST-SSR markers. The molecular analyses revealed a total of 133 alleles ranging from 142 to 330 bp with a mean number of alleles per locus of 4.29. The mean polymorphic information content (PIC) value was calculated as 0.49, and the EST-SSRs in loci S5, S6 and S116 were of the most informative PICs. A dendrogram based on Nei's genetic distance matrix revealed that breeding lines were grouped in two main clusters. Genetic distances were higher between GP6/GP11, GP4/GP11 and GP5/GP8 accessions which could be further used in crop improvement studies for developing wider genetic diversity.
英文关键词	genetic diversity microsatellite molecular selection
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000562145500001
WOS关键词	SIMPLE SEQUENCE REPEAT ; GENETIC DIVERSITY ANALYSIS ; POPULATION-STRUCTURE ; L-CICERA ; POLYMORPHISM ; VARIABILITY ; IMPROVEMENT ; CULTIVARS ; PROTEIN ; YIELD
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325793
作者单位	[Arslan, Mehmet; Basak, Merve; Aksu, Elcin; Uzun, Bulent; Yol, Engin] Akdeniz Univ, Fac Agr, Dept Field Crops, Antalya, Turkey
推荐引用方式 GB/T 7714	Arslan, Mehmet,Basak, Merve,Aksu, Elcin,et al. Genotyping of Low beta-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers[J],2020,63.
APA	Arslan, Mehmet,Basak, Merve,Aksu, Elcin,Uzun, Bulent,&Yol, Engin.(2020).Genotyping of Low beta-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers.BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY,63.
MLA	Arslan, Mehmet,et al."Genotyping of Low beta-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers".BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY 63(2020).