干旱区生态环境知识资源中心(Arid): Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China

Arid

DOI	10.1016/j.apsoil.2020.103644
	Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China
	Du, Shuai; Shen, Ju-Pei; Sun, Yi-Fei; Bai, Yong-Fei; Pan, Hong; Li, Yong; Wang, Zhong-Wu; Han, Guo-Dong; Zhang, Li-Mei; He, Ji-Zheng
通讯作者	Shen, JP
来源期刊	APPLIED SOIL ECOLOGY
ISSN	0929-1393
EISSN	1873-0272
出版年	2020
卷号	155
英文摘要	Long-term livestock grazing often comes with inputs of manure and urine of animals to grassland soils and can influence the microbial communities. However, the effects of grazing on the diversity and abundance of soil antibiotic resistance genes (ARGs) are still obscure. Here we profiled soil resistome using high-throughput quantitative PCR analysis from desert and typical grasslands (Siziwang and Xilingol steppe, respectively) in Inner Mongolia, each with four grazing intensities (no grazing, light grazing, moderate grazing, and heavy grazing). Totally, 54 and 39 ARGs were detected in the Siziwang steppe and the Xilingol steppe, respectively. The relative abundance of ARGs significantly decreased in the Siziwang steppe or remained unchanged in the Xilingol steppe along the gradient of increasing grazing intensity. Mobile genetic elements (MGEs) were significantly and positively correlated with ARGs in the Siziwang steppe but not in the Xilingol steppe. Structural equation model revealed that grazing indirectly affected ARGs through its impact on MGEs, bacterial diversity, and bacterial community composition. These findings suggest that livestock grazing can impact soil resistome via regulating microbiome in grassland soils. However, grazing intensity has not resulted in a significant impact on the soil resistomes in these desert and typical grasslands.
英文关键词	Grassland soil Grazing intensity Antibiotic resistance gene Mobile genetic elements Bacterial community
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000560743700019
WOS关键词	RESISTANCE GENES ; BACTERIAL COMMUNITIES ; MICROBIAL COMMUNITY ; NITROGEN ADDITION ; CATTLE ; SUCCESSION ; INTENSITY ; RESPONSES ; IMPACTS ; MANURES
WOS类目	Soil Science
WOS研究方向	Agriculture
来源机构	中国农业大学 ; 中国科学院植物研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325697
作者单位	[Du, Shuai; Shen, Ju-Pei; Zhang, Li-Mei] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China; [Du, Shuai; Shen, Ju-Pei; Bai, Yong-Fei; He, Ji-Zheng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Sun, Yi-Fei] China Agr Univ, Coll Land Sci & Technol, Beijing 100193, Peoples R China; [Bai, Yong-Fei] Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China; [Pan, Hong] Shandong Agr Univ, Coll Resources & Environm Sci, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An 271018, Shandong, Peoples R China; [Li, Yong] Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Zhejiang Prov Key Lab Agr Resources & Environm, Hangzhou 310058, Peoples R China; [Wang, Zhong-Wu; Han, Guo-Dong] Inner Mongolia Agr Univ, Dept Grassland Sci, Coll Ecol & Environm Sci, Hohhot 010018, Peoples R China; [Wang, Zhong-Wu; Han, Guo-Dong] Inner Mongolia Agr Univ, Inner Mongolia Key Lab Management & Utilizat, Key Lab F...
推荐引用方式 GB/T 7714	Du, Shuai,Shen, Ju-Pei,Sun, Yi-Fei,et al. Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China[J]. 中国农业大学, 中国科学院植物研究所,2020,155.
APA	Du, Shuai.,Shen, Ju-Pei.,Sun, Yi-Fei.,Bai, Yong-Fei.,Pan, Hong.,...&He, Ji-Zheng.(2020).Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China.APPLIED SOIL ECOLOGY,155.
MLA	Du, Shuai,et al."Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China".APPLIED SOIL ECOLOGY 155(2020).