干旱区生态环境知识资源中心(Arid): Genome-scale metabolic models of Microbacterium species isolated from a high altitude desert environment

Arid

DOI	10.1038/s41598-020-62130-8
	Genome-scale metabolic models of Microbacterium species isolated from a high altitude desert environment
	Mandakovic, Dinka; Cintolesi, Angela; Maldonado, Jonathan; Mendoza, Sebastian N.; Aite, Meziane; Gaete, Alexis; Saitua, Francisco; Allende, Miguel; Cambiazo, Veronica; Siegel, Anne; Maass, Alejandro; Gonzalez, Mauricio; Latorre, Mauricio
通讯作者	Gonzalez, M ; Latorre, M
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2020
卷号	10 期号:1
英文摘要	The Atacama Desert is the most arid desert on Earth, focus of important research activities related to microbial biodiversity studies. In this context, metabolic characterization of arid soil bacteria is crucial to understand their survival strategies under extreme environmental stress. We investigated whether strain-specific features of two Microbacterium species were involved in the metabolic ability to tolerate/adapt to local variations within an extreme desert environment. Using an integrative systems biology approach we have carried out construction and comparison of genome-scale metabolic models (GEMs) of two Microbacterium sp., CGR1 and CGR2, previously isolated from physicochemically contrasting soil sites in the Atacama Desert. Despite CGR1 and CGR2 belong to different phylogenetic clades, metabolic pathways and attributes are highly conserved in both strains. However, comparison of the GEMs showed significant differences in the connectivity of specific metabolites related to pH tolerance and CO2 production. The latter is most likely required to handle acidic stress through decarboxylation reactions. We observed greater GEM connectivity within Microbacterium sp. CGR1 compared to CGR2, which is correlated with the capacity of CGR1 to tolerate a wider pH tolerance range. Both metabolic models predict the synthesis of pigment metabolites (beta -carotene), observation validated by HPLC experiments. Our study provides a valuable resource to further investigate global metabolic adaptations of bacterial species to grow in soils with different abiotic factors within an extreme environment.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000560406800008
WOS关键词	SP NOV. ; ORLA-JENSEN ; BACTERIAL ; PH ; SEQUENCE ; GROWTH ; GENES ; RECLASSIFICATION ; RECONSTRUCTION ; BIOSYNTHESIS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325668
作者单位	[Mandakovic, Dinka; Maldonado, Jonathan; Gaete, Alexis; Cambiazo, Veronica; Gonzalez, Mauricio; Latorre, Mauricio] INTA Univ Chile, Lab Bioinformat & Expres Gen, Santiago 5524, Chile; [Mandakovic, Dinka; Cintolesi, Angela; Maldonado, Jonathan; Mendoza, Sebastian N.; Gaete, Alexis; Allende, Miguel; Cambiazo, Veronica; Maass, Alejandro; Gonzalez, Mauricio; Latorre, Mauricio] Fondap Ctr Genome Regulat CGR, Ave Blanco Encalada 2085, Santiago, Chile; [Cintolesi, Angela; Mendoza, Sebastian N.; Maass, Alejandro; Latorre, Mauricio] Univ Chile, Ctr Math Modeling, Math, Santiago, Chile; [Mendoza, Sebastian N.] Vrije Univ Amsterdam, Syst Biol Lab, Amsterdam Inst Mol Med & Syst, De Boelelaan 1108, NL-1081 HV Amsterdam, Netherlands; [Aite, Meziane; Siegel, Anne] CNRS, IRISA, UMR 6074, Rennes, France; [Aite, Meziane; Siegel, Anne] INRIA, DylissTeam, Ctr Rennes Bretagne Atlantique, Rennes, France; [Saitua, Francisco] Pontificia Univ Catolica Chile, Sch Engn, Dept Chem & Bioproc Engn, Ave Vicuna Mackenna 4860, Santiago, ...
推荐引用方式 GB/T 7714	Mandakovic, Dinka,Cintolesi, Angela,Maldonado, Jonathan,et al. Genome-scale metabolic models of Microbacterium species isolated from a high altitude desert environment[J],2020,10(1).
APA	Mandakovic, Dinka.,Cintolesi, Angela.,Maldonado, Jonathan.,Mendoza, Sebastian N..,Aite, Meziane.,...&Latorre, Mauricio.(2020).Genome-scale metabolic models of Microbacterium species isolated from a high altitude desert environment.SCIENTIFIC REPORTS,10(1).
MLA	Mandakovic, Dinka,et al."Genome-scale metabolic models of Microbacterium species isolated from a high altitude desert environment".SCIENTIFIC REPORTS 10.1(2020).