干旱区生态环境知识资源中心(Arid): CrebAincreases secretory capacity through direct transcriptional regulation of the secretory machinery, a subset of secretory cargo, and other key regulators

Arid

DOI	10.1111/tra.12753
	CrebAincreases secretory capacity through direct transcriptional regulation of the secretory machinery, a subset of secretory cargo, and other key regulators
	Johnson, Dorothy M.; Wells, Michael B.; Fox, Rebecca; Lee, Joslynn S.; Loganathan, Rajprasad; Levings, Daniel; Bastien, Abigail; Slattery, Matthew; Andrew, Deborah J.
通讯作者	Andrew, DJ
来源期刊	TRAFFIC
ISSN	1398-9219
EISSN	1600-0854
出版年	2020
卷号	21 期号:9 页码:560-577
英文摘要	Specialization of many cells, including the acinar cells of the salivary glands and pancreas, milk-producing cells of mammary glands, mucus-secreting goblet cells, antibody-producing plasma cells, and cells that generate the dense extracellular matrices of bone and cartilage, requires scaling up both secretory machinery and cell-type specific secretory cargo. Using tissue-specific genome-scale analyses, we determine how increases in secretory capacity are coordinated with increases in secretory load in the Drosophila salivary gland (SG), an ideal model for gaining mechanistic insight into the functional specialization of secretory organs. Our findings show that CrebA, a bZIP transcription factor, directly binds genes encoding the core secretory machinery, including protein components of the signal recognition particle and receptor, ER cargo translocators, Cop I and Cop II vesicles, as well as the structural proteins and enzymes of these organelles. CrebA directly binds a subset of SG cargo genes and CrebA binds and boosts expression of Sage, a SG-specific transcription factor essential for cargo expression. To further enhance secretory output, CrebA binds and activatesXbp1andTudor-SN. Thus, CrebA directly upregulates the machinery of secretion and additional factors to increase overall secretory capacity in professional secretory cells; concomitant increases in cargo are achieved both directly and indirectly.
英文关键词	ChIP-seq CrebA Drosophila sage salivary gland secretory capacity secretory cargo transcription factor TSN Xbp1
类型	Article
语种	英语
开放获取类型	Green Accepted, Bronze
收录类别	SCI-E
WOS记录号	WOS:000560248200001
WOS关键词	STRESS TRANSDUCER OASIS ; SALIVARY-GLAND ; DCREB-A ; DROSOPHILA ; GENE ; EXPRESSION ; PACKAGE ; PATHWAY
WOS类目	Cell Biology
WOS研究方向	Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325655
作者单位	[Johnson, Dorothy M.; Wells, Michael B.; Fox, Rebecca; Loganathan, Rajprasad; Bastien, Abigail; Andrew, Deborah J.] Johns Hopkins Univ, Dept Cell Biol, Sch Med, 725 N Wolfe St, Baltimore, MD 21205 USA; [Lee, Joslynn S.; Levings, Daniel; Slattery, Matthew] Univ Minnesota, Sch Med, Dept Biomed Sci, Duluth, MN 55812 USA
推荐引用方式 GB/T 7714	Johnson, Dorothy M.,Wells, Michael B.,Fox, Rebecca,et al. CrebAincreases secretory capacity through direct transcriptional regulation of the secretory machinery, a subset of secretory cargo, and other key regulators[J],2020,21(9):560-577.
APA	Johnson, Dorothy M..,Wells, Michael B..,Fox, Rebecca.,Lee, Joslynn S..,Loganathan, Rajprasad.,...&Andrew, Deborah J..(2020).CrebAincreases secretory capacity through direct transcriptional regulation of the secretory machinery, a subset of secretory cargo, and other key regulators.TRAFFIC,21(9),560-577.
MLA	Johnson, Dorothy M.,et al."CrebAincreases secretory capacity through direct transcriptional regulation of the secretory machinery, a subset of secretory cargo, and other key regulators".TRAFFIC 21.9(2020):560-577.