干旱区生态环境知识资源中心(Arid): Multiomics approach unravels fertility transition in a pigeonpea line for a two-line hybrid system

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DOI	10.1002/tpg2.20028
	Multiomics approach unravels fertility transition in a pigeonpea line for a two-line hybrid system
	Pazhamala, Lekha T.; Chaturvedi, Palak; Bajaj, Prasad; Srikanth, Sandhya; Ghatak, Arindam; Chitikineni, Annapurna; Bellaire, Anke; Hingane, Anupama; Kumar, C. V. Sameer; Saxena, K. B.; Weckwerth, Wolfram; Saxena, Rachit K.; Varshney, Rajeev K.
通讯作者	Weckwerth, W
来源期刊	PLANT GENOME
EISSN	1940-3372
出版年	2020
卷号	13 期号:2
英文摘要	Pigeonpea [Cajanus cajan (L.) Millsp.] is a pulse crop cultivated in the semi-arid regions of Asia and Africa. It is a rich source of protein and capable of alleviating malnutrition, improving soil health and the livelihoods of small-holder farmers. Hybrid breeding has provided remarkable improvements for pigeonpea productivity, but owing to a tedious and costly seed production system, an alternative two-line hybrid technology is being explored. In this regard, an environment-sensitive male sterile line has been characterized as a thermosensitive male sterile line in pigeonpea precisely responding to day temperature. The male sterile and fertile anthers from five developmental stages were studied by integrating transcriptomics, proteomics and metabolomics supported by precise phenotyping and scanning electron microscopic study. Spatio-temporal analysis of anther transcriptome and proteome revealed 17 repressed DEGs/DEPs in sterile anthers that play a critical role in normal cell wall morphogenesis and tapetal cell development. The male fertility to sterility transition was mainly due to a perturbation in auxin homeostasis, leading to impaired cell wall modification and sugar transport. Limited nutrient utilization thus leads to microspore starvation in response to moderately elevated day temperature which could be restored with auxin-treatment in the male sterile line. Our findings outline a molecular mechanism that underpins fertility transition responses thereby providing a process-oriented two-line hybrid breeding framework for pigeonpea.
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000559970200011
WOS关键词	MALE-STERILITY SYSTEM ; POLLEN MOTHER CELL ; GENE ; RNA ; IDENTIFICATION ; ARABIDOPSIS ; PROTEINS ; ANTHER ; RICE ; QUANTIFICATION
WOS类目	Plant Sciences ; Genetics & Heredity
WOS研究方向	Plant Sciences ; Genetics & Heredity
来源机构	International Crops Research Institute for the Semi-Arid Tropics ; University of Western Australia
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325639
作者单位	[Pazhamala, Lekha T.; Bajaj, Prasad; Srikanth, Sandhya; Chitikineni, Annapurna; Saxena, Rachit K.; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Ctr Excellence Genom & Syst Biol, Hyderabad 502324, India; [Chaturvedi, Palak; Ghatak, Arindam; Bellaire, Anke; Weckwerth, Wolfram] Univ Vienna, Mol Syst Biol MOSYS, Dept Funct & Evolutionary Ecol, Fac Life Sci, Althanstr 14, A-1090 Vienna, Austria; [Bellaire, Anke] Univ Vienna, Dept Bot & Biodivers Res, Rennweg 14, A-1030 Vienna, Austria; [Hingane, Anupama; Kumar, C. V. Sameer; Saxena, K. B.] Int Crops Res Inst Semi Arid Trop, Crop Improvement Theme, Hyderabad 502324, India; [Weckwerth, Wolfram] Univ Vienna, Vienna Metab Ctr VIME, Vienna, Austria; [Varshney, Rajeev K.] Univ Western Australia, Inst Agr, 35 Stirling Highway, Crawley, WA 6009, Australia
推荐引用方式 GB/T 7714	Pazhamala, Lekha T.,Chaturvedi, Palak,Bajaj, Prasad,et al. Multiomics approach unravels fertility transition in a pigeonpea line for a two-line hybrid system[J]. International Crops Research Institute for the Semi-Arid Tropics, University of Western Australia,2020,13(2).
APA	Pazhamala, Lekha T..,Chaturvedi, Palak.,Bajaj, Prasad.,Srikanth, Sandhya.,Ghatak, Arindam.,...&Varshney, Rajeev K..(2020).Multiomics approach unravels fertility transition in a pigeonpea line for a two-line hybrid system.PLANT GENOME,13(2).
MLA	Pazhamala, Lekha T.,et al."Multiomics approach unravels fertility transition in a pigeonpea line for a two-line hybrid system".PLANT GENOME 13.2(2020).