干旱区生态环境知识资源中心(Arid): Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase

Arid

DOI	10.1016/j.ejbt.2020.05.003
	Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase
	Li, Xianjun; Wang, Junhuan; Wu, Wei; Jia, Yang; Fan, Shuanghu; Hlaing, Thet Su; Khokhar, Ibatsam; Yan, Yanchun
通讯作者	Yan, YC
来源期刊	ELECTRONIC JOURNAL OF BIOTECHNOLOGY
ISSN	0717-3458
出版年	2020
卷号	46 页码:38-49
英文摘要	Background: Quizalofop-p-ethyl (QPE), a unitary R configuration aromatic oxyphenoxypropionic acid ester (AOPP) herbicide, was widely used and had led to detrimental environmental effects. For finding the QPE-degrading bacteria and promoting the biodegradation of QPE, a series of studies were carried out. Results: A QPE-degrading bacterial strain YC-XJ1 was isolated from desert soil and identified as Methylobacterium populi, which could degrade QPE with methanol by cometabolism. Ninety-seven percent of QPE (50mg/L) could be degraded within 72 h under optimum biodegradation condition of 35 degrees C and pH 8.0. The maximum degradation rate of QPE was 1.4 mg/L/h, and the strain YC-XJ1 exhibited some certain salinity tolerance. Two novel metabolites, 2-hydroxy-6-chloroquinoxaline and quinoxaline, were found by high-performance liquid chromatography/mass spectroscopy analysis. The metabolic pathway of QPE was predicted. The catalytic efficiency of strain YC-XJ1 toward different AOPPs herbicides in descending order was as follows: haloxyfop-p-methyl approximate to diclofop-methyl approximate to fluazifop-p-butyl N clodinafop-propargyl N cyhalofop-butyl N quizalofop-p-ethyl N fenoxaprop-p-ethyl N propaquizafop N quizalofop-p-tefuryl. The genome of strain YC-XJ1 was sequenced using a combination of PacBio RS II and Illumina platforms. According to the annotation result, one alpha/beta hydrolase gene was selected and named qpeh1, for which QPE-degrading function has obtained validation. Based on the phylogenetic analysis and multiple sequence alignment with other QPE-degrading esterases reported previously, the QPEH1 was clustered with esterase family V. Conclusion: M. populi YC-XJ1 could degrade QPE with a novel pathway, and the qpeh1 gene was identified as one of QPE-degrading esterase gene. (C) 2020 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.
英文关键词	Aromatic oxyphenoxypropionic acid esters Bioremediation Co-metabolic biodegradation Desert soil Genome sequencing Grassy weeds Herbicide Methylobacterium populi Novel metabolites Quizalofop-p-ethyl alpha/beta hydrolase
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	SCI-E
WOS记录号	WOS:000557952300006
WOS关键词	CYHALOFOP-BUTYL ; DICHLOROMETHANE ; DEGRADATION ; CLONING ; HERBICIDES
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325538
作者单位	[Li, Xianjun; Wang, Junhuan; Wu, Wei; Jia, Yang; Fan, Shuanghu; Hlaing, Thet Su; Khokhar, Ibatsam; Yan, Yanchun] Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Li, Xianjun,Wang, Junhuan,Wu, Wei,et al. Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase[J],2020,46:38-49.
APA	Li, Xianjun.,Wang, Junhuan.,Wu, Wei.,Jia, Yang.,Fan, Shuanghu.,...&Yan, Yanchun.(2020).Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,46,38-49.
MLA	Li, Xianjun,et al."Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 46(2020):38-49.