干旱区生态环境知识资源中心(Arid): Anastatica hierochuntica (L.) methanolic and aqueous extracts exert antiproliferative effects through the induction of apoptosis in MCF-7 breast cancer cells

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DOI	10.1016/j.jsps.2020.06.020
	Anastatica hierochuntica (L.) methanolic and aqueous extracts exert antiproliferative effects through the induction of apoptosis in MCF-7 breast cancer cells
	Rameshbabu, Saranya; Messaoudi, Safia A.; Alehaideb, Zeyad Ibrahim; Ali, Mohammed Syed; Venktraman, Anuradha; Alajmi, Hala; Al-Eidi, Hamad; Matou-Nasri, Sabine
通讯作者	Matou-Nasri, S
来源期刊	SAUDI PHARMACEUTICAL JOURNAL
ISSN	1319-0164
EISSN	2213-7475
出版年	2020
卷号	28 期号:8 页码:985-993
英文摘要	Breast cancer therapy using anticancer bioactive compounds derived from natural products as adjuvant treatment has gained recognition due to expensive and toxic conventional chemotherapeutic drugs. The whole plant of Anastatica hierochuntica (L.) (A. hierochuntica) has been investigated for its pharmacologically important anticancer properties but without categorizing the biological activities of the plant parts. We assessed the anticancer potential of different parts of A. hierochuntica (seeds, stems and leaves) and explored their mechanisms of action using the human breast cancer cell line, MCF-7. Currently, we investigated the antiproliferative effects of methanolic (MSD, MST, ML) and aqueous (ASD, AST, AL) extracts of A. hierochuntica plant parts on the MCF-7 cells using cell viability assays. Flow cytometry, Western Blot, DNA fragmentation, and gene expression assays were performed to evaluate apoptosis and cell cycle regulatory proteins. The results indicate that the methanolic and aqueous extracts decreased MCF-7 cell viability in a dose-dependent manner. The induction of apoptosis was observed in all the methanolic and aqueous-treated MCF-7 cells. The cell death process was confirmed by the visualization of DNA fragmentation and cleavage of the intrinsic apoptotic pathways, caspase-9 and caspase-3, the key enzyme causing apoptosis hallmarks. In addition, the most pro-apoptotic extracts, ASD and ML, up-regulated the expression of pro-apoptotic Bax, tumor suppressor TP53 genes and the cyclin inhibitor CDKN1A gene. In conclusion, of the aqueous and methanolic extracts of A. hierochuntica plant parts exerting antiproliferative effects through the induction of apoptosis in breast cancer MCF-7 cells, ASD and ML extracts were the most promising natural-based drugs for the treatment of breast cancer. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
英文关键词	Anastatica hierochuntica Apoptosis P53 Cell cycle Breast cancer Natural products
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000557427600011
WOS关键词	IN-VITRO ; CYCLE ; CDKS ; P53 ; DESERT ; BCL-2
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325510
作者单位	[Rameshbabu, Saranya; Venktraman, Anuradha] Mohamed Sathak Coll Arts & Sci, Post Grad & Res Dept Biochem, Chennai, Tamil Nadu, India; [Messaoudi, Safia A.] Naif Arab Univ Secur Sci, Coll Criminal Justice, Dept Forens Sci, Riyadh, Saudi Arabia; [Alehaideb, Zeyad Ibrahim; Al-Eidi, Hamad; Matou-Nasri, Sabine] Minist Natl Guard Hlth Affairs, Med Genom Res Dept, King Abdullah Int Med Res Ctr, Cell & Gene Therapy Grp, Riyadh, Saudi Arabia; [Alajmi, Hala] Minist Natl Guard Hlth Affairs, King Abdullah Int Med Res Ctr, Biobank, Riyadh, Saudi Arabia; [Alehaideb, Zeyad Ibrahim; Matou-Nasri, Sabine] King Saud Bin Abdulaziz Univ Hlth Sci, Riyadh, Saudi Arabia; [Ali, Mohammed Syed] Mohamed Sathak Coll Arts & Sci, Dept Biotechnol, Chennai, Tamil Nadu, India
推荐引用方式 GB/T 7714	Rameshbabu, Saranya,Messaoudi, Safia A.,Alehaideb, Zeyad Ibrahim,et al. Anastatica hierochuntica (L.) methanolic and aqueous extracts exert antiproliferative effects through the induction of apoptosis in MCF-7 breast cancer cells[J],2020,28(8):985-993.
APA	Rameshbabu, Saranya.,Messaoudi, Safia A..,Alehaideb, Zeyad Ibrahim.,Ali, Mohammed Syed.,Venktraman, Anuradha.,...&Matou-Nasri, Sabine.(2020).Anastatica hierochuntica (L.) methanolic and aqueous extracts exert antiproliferative effects through the induction of apoptosis in MCF-7 breast cancer cells.SAUDI PHARMACEUTICAL JOURNAL,28(8),985-993.
MLA	Rameshbabu, Saranya,et al."Anastatica hierochuntica (L.) methanolic and aqueous extracts exert antiproliferative effects through the induction of apoptosis in MCF-7 breast cancer cells".SAUDI PHARMACEUTICAL JOURNAL 28.8(2020):985-993.