干旱区生态环境知识资源中心(Arid): Analysis and characterization of the Aldehyde dehydrogenase (ALDH) gene superfamily in the desert moss Syntrichia caninervis in response to abiotic stress

Arid

DOI	10.1016/j.envexpbot.2020.104176
	Analysis and characterization of the Aldehyde dehydrogenase (ALDH) gene superfamily in the desert moss Syntrichia caninervis in response to abiotic stress
	Wang, Jiancheng; Yang, Honglan; Gao, Bei; Bozorov, Tohir A.; Li, Xiaoshuang; Zhang, Daoyuan; Wood, Andrew J.
通讯作者	Zhang, DY
来源期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
EISSN	1873-7307
出版年	2020
卷号	178
英文摘要	Syntrichia caninervis is desiccation-tolerant moss and a dominant species in biological soil crusts in the Gurbantunggut desert of Northwestern China. S. caninervis is being developed as a model moss for studying the molecular mechanisms of desiccation tolerance (DT) and as a source for stress-related genes germplasm. Aldehyde dehydrogenases (ALDHs) are a set of NAD (P) + dependent enzymes that oxidize a wide range of endogenous and exogenous aromatic and aliphatic aldehydes into their corresponding non-toxic carboxylic acids. In the current study, we identified 15 ALDH genes from the moss S.caninervis, grouped them into eleven families based upon clearly established criteria. As determined by RT-qPCR, transcript abundance of ScALDH genes respond to drought, salinity, cold, and/or ABA treatment. ScALDH5F, ScALDH6B1, ScALDH7B1, ScALDH11A1 and ScALDH21A1 exhibited consistent up-regulation as compared to other transcripts. We selected four genes (ScALDH2B2, ScALDH10A1, ScALDH11A1 and ScALDH21A1) based upon sequence characteristics and qRT-PCR profiles, and subsequently cloned and transformed to E. coll. strain where the transgenes conferred varying degree of drought and salt tolerance. The four purified ScALDH proteins indicated enzyme activities with aldehyde of C6-C9 carbon chain as substrates were more suitable than that of C1-C2 carbon chain, especially unsaturated aldehyde, all have their own special substrates.
英文关键词	Aldehyde dehydrogenase (ALDH) Gene family Tolerance Abiotic stress Syntrichia caninervis
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000556869600030
WOS关键词	GENOME-WIDE IDENTIFICATION ; ARABIDOPSIS-THALIANA ; GURBANTUNGGUT DESERT ; PLANTS ; OVEREXPRESSION ; DROUGHT ; NOMENCLATURE ; DESICCATION ; EXPRESSION ; SALT
WOS类目	Plant Sciences ; Environmental Sciences
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325479
作者单位	[Wang, Jiancheng; Yang, Honglan; Gao, Bei; Bozorov, Tohir A.; Li, Xiaoshuang; Zhang, Daoyuan] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China; [Bozorov, Tohir A.] Uzbekistan Natl Univ, Fac Biol, Univ Str 4, Tashkent 100174, Uzbekistan; [Wood, Andrew J.] Southern Illinois Univ, Sch Biol Sci, Carbondale, IL 62901 USA
推荐引用方式 GB/T 7714	Wang, Jiancheng,Yang, Honglan,Gao, Bei,et al. Analysis and characterization of the Aldehyde dehydrogenase (ALDH) gene superfamily in the desert moss Syntrichia caninervis in response to abiotic stress[J]. 中国科学院新疆生态与地理研究所,2020,178.
APA	Wang, Jiancheng.,Yang, Honglan.,Gao, Bei.,Bozorov, Tohir A..,Li, Xiaoshuang.,...&Wood, Andrew J..(2020).Analysis and characterization of the Aldehyde dehydrogenase (ALDH) gene superfamily in the desert moss Syntrichia caninervis in response to abiotic stress.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,178.
MLA	Wang, Jiancheng,et al."Analysis and characterization of the Aldehyde dehydrogenase (ALDH) gene superfamily in the desert moss Syntrichia caninervis in response to abiotic stress".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 178(2020).