干旱区生态环境知识资源中心(Arid): Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle

Arid

DOI	10.1007/s00360-020-01296-z
	Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle
	Marciniak, Pawel; Urbanski, Arkadiusz; Lubawy, Jan; Szymczak, Monika; Pacholska-Bogalska, Joanna; Chowanski, Szymon; Kuczer, Mariola; Rosinski, Grzegorz
通讯作者	Marciniak, P
来源期刊	JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
ISSN	0174-1578
EISSN	1432-136X
出版年	2020
卷号	190 期号:5 页码:521-534
英文摘要	Neuropeptides of short neuropeptides F family (sNPF) have been identified in various arthropods. They are pleiotropic neuromolecules which so far have been mainly associated with regulation of feeding and metabolism, as well as growth and development, locomotion, circadian rhythm or learning and memory. Here, we describe the effects of Tenebrionid sNPF peptide (SGRSPSLRLRFa) on various aspects of the male reproductive physiology in theTenebrio molitorbeetle. We identified in silico the putative sNPF receptor Tenmo-sNPFR. Based on RT-PCR technique, it was shown that the receptor might be present in the male reproductive tissues of this beetle. The analysis of receptor amino acid sequence showed that it is similar to other beetle sNPFRs, as well as other insect species, and belongs rhodopsin-like G-protein-coupled receptors (GPCRs). Injections of Trica-sNPF and its shorter form Trica-sNPF((4-11))caused differentiated effects inT. molitormale reproductive tissues. After 24 h post injections, the peptides decreased the concentration of the soluble protein fraction in testes of 4- and 8-day-old beetles as well as the dry mass of these organs but only in 8-day-old individuals. The same effects were shown with regard to accessory glands. Both peptides decrease the concentration of the soluble protein fraction but do not affect the dry mass of this organ. Furthermore, injections of Trica-sNPF at the 10(-7) M concentration decrease the total sperm number in the reproductive system. Surprisingly, the same concentration of the shorter form, Trica-sNPF((4-11))increased the sperm number. It was also shown that both peptides in different manner influence contractions of ejaculatory duct. The data presented in this article give new evidence that sNPFs are involved in the regulation of reproductive events in beetles, which might be the part of a larger neuropeptide network combining feeding, growth and development with the physiology of reproduction.
英文关键词	Short neuropeptide F Reproduction Testes GPCR Beetle Tenebrio molitor
类型	Article
语种	英语
开放获取类型	Other Gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000555737700002
WOS关键词	IMPORTED FIRE ANT ; NPF-I PEPTIDE ; DROSOPHILA-MELANOGASTER ; NEUROHORMONE GPCRS ; TENEBRIO-MOLITOR ; DESERT LOCUST ; RECEPTOR ; SNPF ; IMMUNOLOCALIZATION ; IDENTIFICATION
WOS类目	Physiology ; Zoology
WOS研究方向	Physiology ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325399
作者单位	[Marciniak, Pawel; Urbanski, Arkadiusz; Lubawy, Jan; Szymczak, Monika; Pacholska-Bogalska, Joanna; Chowanski, Szymon; Rosinski, Grzegorz] Adam Mickiewicz Univ, Dept Anim Physiol & Dev, Uniwersytetu Poznanskiego 6 St, PL-61614 Poznan, Poland; [Urbanski, Arkadiusz] HiProMine SA, Poznanska 8 St, PL-62023 Robakowo, Poland; [Kuczer, Mariola] Univ Wroclaw, Fac Chem, F Foliot Curie 14 St, PL-50383 Wroclaw, Poland
推荐引用方式 GB/T 7714	Marciniak, Pawel,Urbanski, Arkadiusz,Lubawy, Jan,et al. Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle[J],2020,190(5):521-534.
APA	Marciniak, Pawel.,Urbanski, Arkadiusz.,Lubawy, Jan.,Szymczak, Monika.,Pacholska-Bogalska, Joanna.,...&Rosinski, Grzegorz.(2020).Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle.JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY,190(5),521-534.
MLA	Marciniak, Pawel,et al."Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle".JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY 190.5(2020):521-534.