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DOI | 10.1007/s00360-020-01296-z |
Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle | |
Marciniak, Pawel; Urbanski, Arkadiusz; Lubawy, Jan; Szymczak, Monika; Pacholska-Bogalska, Joanna; Chowanski, Szymon; Kuczer, Mariola; Rosinski, Grzegorz | |
通讯作者 | Marciniak, P |
来源期刊 | JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
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ISSN | 0174-1578 |
EISSN | 1432-136X |
出版年 | 2020 |
卷号 | 190期号:5页码:521-534 |
英文摘要 | Neuropeptides of short neuropeptides F family (sNPF) have been identified in various arthropods. They are pleiotropic neuromolecules which so far have been mainly associated with regulation of feeding and metabolism, as well as growth and development, locomotion, circadian rhythm or learning and memory. Here, we describe the effects of Tenebrionid sNPF peptide (SGRSPSLRLRFa) on various aspects of the male reproductive physiology in theTenebrio molitorbeetle. We identified in silico the putative sNPF receptor Tenmo-sNPFR. Based on RT-PCR technique, it was shown that the receptor might be present in the male reproductive tissues of this beetle. The analysis of receptor amino acid sequence showed that it is similar to other beetle sNPFRs, as well as other insect species, and belongs rhodopsin-like G-protein-coupled receptors (GPCRs). Injections of Trica-sNPF and its shorter form Trica-sNPF((4-11))caused differentiated effects inT. molitormale reproductive tissues. After 24 h post injections, the peptides decreased the concentration of the soluble protein fraction in testes of 4- and 8-day-old beetles as well as the dry mass of these organs but only in 8-day-old individuals. The same effects were shown with regard to accessory glands. Both peptides decrease the concentration of the soluble protein fraction but do not affect the dry mass of this organ. Furthermore, injections of Trica-sNPF at the 10(-7) M concentration decrease the total sperm number in the reproductive system. Surprisingly, the same concentration of the shorter form, Trica-sNPF((4-11))increased the sperm number. It was also shown that both peptides in different manner influence contractions of ejaculatory duct. The data presented in this article give new evidence that sNPFs are involved in the regulation of reproductive events in beetles, which might be the part of a larger neuropeptide network combining feeding, growth and development with the physiology of reproduction. |
英文关键词 | Short neuropeptide F Reproduction Testes GPCR Beetle Tenebrio molitor |
类型 | Article |
语种 | 英语 |
开放获取类型 | Other Gold, Green Published |
收录类别 | SCI-E |
WOS记录号 | WOS:000555737700002 |
WOS关键词 | IMPORTED FIRE ANT ; NPF-I PEPTIDE ; DROSOPHILA-MELANOGASTER ; NEUROHORMONE GPCRS ; TENEBRIO-MOLITOR ; DESERT LOCUST ; RECEPTOR ; SNPF ; IMMUNOLOCALIZATION ; IDENTIFICATION |
WOS类目 | Physiology ; Zoology |
WOS研究方向 | Physiology ; Zoology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/325399 |
作者单位 | [Marciniak, Pawel; Urbanski, Arkadiusz; Lubawy, Jan; Szymczak, Monika; Pacholska-Bogalska, Joanna; Chowanski, Szymon; Rosinski, Grzegorz] Adam Mickiewicz Univ, Dept Anim Physiol & Dev, Uniwersytetu Poznanskiego 6 St, PL-61614 Poznan, Poland; [Urbanski, Arkadiusz] HiProMine SA, Poznanska 8 St, PL-62023 Robakowo, Poland; [Kuczer, Mariola] Univ Wroclaw, Fac Chem, F Foliot Curie 14 St, PL-50383 Wroclaw, Poland |
推荐引用方式 GB/T 7714 | Marciniak, Pawel,Urbanski, Arkadiusz,Lubawy, Jan,et al. Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle[J],2020,190(5):521-534. |
APA | Marciniak, Pawel.,Urbanski, Arkadiusz.,Lubawy, Jan.,Szymczak, Monika.,Pacholska-Bogalska, Joanna.,...&Rosinski, Grzegorz.(2020).Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle.JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY,190(5),521-534. |
MLA | Marciniak, Pawel,et al."Short neuropeptide F signaling regulates functioning of male reproductive system inTenebrio molitorbeetle".JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY 190.5(2020):521-534. |
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