干旱区生态环境知识资源中心(Arid): Indirect regeneration ofFicus caricaby the TCL technique and genetic fidelity evaluation of the regenerated plants using flow cytometry and ISSR

Arid

DOI	10.1007/s11240-020-01903-5
	Indirect regeneration ofFicus caricaby the TCL technique and genetic fidelity evaluation of the regenerated plants using flow cytometry and ISSR
	Abdolinejad, Ruhollah; Shekafandeh, Akhtar; Jowkar, Abolfazl; Gharaghani, Ali; Alemzadeh, Abbas
通讯作者	Shekafandeh, A
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
EISSN	1573-5044
出版年	2020
卷号	143 期号:1 页码:131-144
英文摘要	Key message A rapid and high-efficient in vitro method for mass propagation via callus culture in twoF.caricacultivars was established by using TCL technique for the first time. Flow cytometry and ISSR molecular markers confirmed the clonal identity of regenerants in both cultivars. The fig (Ficus caricaL.), is known as a precious fruit tree for its nutrition and medicinal values, economic importance and for sustainable production in the semi-arid and arid areas. Expanding the cultivation of fig in new vulnerable areas and the breeding programs in fig need a reliable high-efficient system for in vitro morphogenesis to meet future demands. This study was carried to develop an efficient protocol for indirect regeneration ofF.caricaL. cultivars 'Sabz' and 'Torsh' using thin cell layer (TCL) technique. The genetic fidelity of the regenerated plants was also evaluated using flow cytometry technique and ISSR markers. Stem segments of 10 mm in diameter were taken from mature plants, then explants were transversally cut into layers of 0.5-0.8 mm thickness. Callus induction was successful using Murashige and Tucker (MT) medium supplemented with 9.08 mu M TDZ plus 9.8 mu M IBA (IM3 medium) which resulted in 50 +/- 6.11% calli in 'Sabz' cultivar. Morphogenic calli were cut into small pieces and cultured on Murashige and Skoog (MS) medium for shoot development. Maximum shoot regeneration (45%) was observed in 17.68 mu M BAP in combination with 4.54 mu M TDZ and 1.07 mu M NAA (RM2 medium), with an average of 6.9 shoots per explant. Flow cytometry and ISSR molecular marker analyses confirmed the stability of ploidy level and genetic identity of indirectly regenerated plants in both cultivars. The results of this study demonstrate that indirect regeneration ofF.caricaL. by the use of TCL system is a reliable and promising approach for future mass propagation programs as well as possible in vitro breeding objectives.
英文关键词	Fig (Ficus caricaL ) Flow cytometry Molecular markers Morphogenic callus Plant growth regulators
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000555726800001
WOS关键词	GENOME SIZE ANALYSIS ; FICUS-CARICA ; SOMATIC EMBRYOGENESIS ; FIG ; L. ; PROPAGATION ; SEGMENTS ; RAPD
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325398
作者单位	[Abdolinejad, Ruhollah; Shekafandeh, Akhtar; Jowkar, Abolfazl; Gharaghani, Ali] Shiraz Univ, Coll Agr, Dept Hort Sci, Shiraz, Iran; [Alemzadeh, Abbas] Shiraz Univ, Coll Agr, Dept Plant Genet & Prod, Shiraz, Iran
推荐引用方式 GB/T 7714	Abdolinejad, Ruhollah,Shekafandeh, Akhtar,Jowkar, Abolfazl,et al. Indirect regeneration ofFicus caricaby the TCL technique and genetic fidelity evaluation of the regenerated plants using flow cytometry and ISSR[J],2020,143(1):131-144.
APA	Abdolinejad, Ruhollah,Shekafandeh, Akhtar,Jowkar, Abolfazl,Gharaghani, Ali,&Alemzadeh, Abbas.(2020).Indirect regeneration ofFicus caricaby the TCL technique and genetic fidelity evaluation of the regenerated plants using flow cytometry and ISSR.PLANT CELL TISSUE AND ORGAN CULTURE,143(1),131-144.
MLA	Abdolinejad, Ruhollah,et al."Indirect regeneration ofFicus caricaby the TCL technique and genetic fidelity evaluation of the regenerated plants using flow cytometry and ISSR".PLANT CELL TISSUE AND ORGAN CULTURE 143.1(2020):131-144.