干旱区生态环境知识资源中心(Arid): Rapid determination of amoxicillin in porcine tissues by UPLC-MS/MS with internal standard

Arid

DOI	10.1016/j.jfca.2020.103578
	Rapid determination of amoxicillin in porcine tissues by UPLC-MS/MS with internal standard
	Zhang, Caiyun; Zeng, Jiaxiong; Xiong, Wenguang; Zeng, Zhenling
通讯作者	Zeng, ZL
来源期刊	JOURNAL OF FOOD COMPOSITION AND ANALYSIS
ISSN	0889-1575
EISSN	1096-0481
出版年	2020
卷号	92
英文摘要	A rapid, simple and reliable method using amoxicillin-D4 as internal standard (IS) for determination of amoxicillin (AMO) in porcine tissues was developed. The analytical methodology used a phosphate buffer (0.01 M, pH = 6.3) with trichloroacetic acid (50 mg/mL) for extraction and Oasis (R) HLB cartridges for clean-up followed by UPLC-MS/MS analysis. The optimized method was validated according to EU guideline 2002/657/EC and FDA guideline. The LOQ in muscle, fat, liver and kidney were set to 10 mu g/kg which was below the maximum residue limits, while the LOD were set to 5 mu g/kg. Results showed that using AMO-D4 as IS can effectively eliminate relative matrix interference and improve the recovery and precision. A good linearity was observed at the range of 10 - 500 mu g/kg with a correlation coefficients (R-2) larger than 0.99. The mean recoveries were higher than 87.0 %, and the RSDs were lower than 11.2 %. This method could be applied to conveniently analysis the residue of AMO in different porcine tissues.
英文关键词	Amoxicillin UPLC-MS/MS Porcine tissues Maximum residue limits Internal standard Validation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000554847700030
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; BETA-LACTAM ANTIBIOTICS ; MAJOR METABOLITES ; QUANTITATIVE-ANALYSIS ; CHICKEN MUSCLE ; HUMAN PLASMA ; EXTRACTION ; QUANTIFICATION ; STABILITY ; BOVINE
WOS类目	Chemistry, Applied ; Food Science & Technology
WOS研究方向	Chemistry ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325352
作者单位	[Zhang, Caiyun; Zeng, Jiaxiong; Xiong, Wenguang; Zeng, Zhenling] South China Agr Univ, Guangdong Prov Key Lab Vet Pharmaceut Dev & Safet, Guangzhou 510642, Peoples R China; [Zhang, Caiyun; Zeng, Jiaxiong; Xiong, Wenguang; Zeng, Zhenling] Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China; [Zhang, Caiyun; Zeng, Jiaxiong; Xiong, Wenguang; Zeng, Zhenling] South China Agr Univ, Natl Reference Lab Vet Drug Residues, Guangzhou 510642, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Caiyun,Zeng, Jiaxiong,Xiong, Wenguang,et al. Rapid determination of amoxicillin in porcine tissues by UPLC-MS/MS with internal standard[J],2020,92.
APA	Zhang, Caiyun,Zeng, Jiaxiong,Xiong, Wenguang,&Zeng, Zhenling.(2020).Rapid determination of amoxicillin in porcine tissues by UPLC-MS/MS with internal standard.JOURNAL OF FOOD COMPOSITION AND ANALYSIS,92.
MLA	Zhang, Caiyun,et al."Rapid determination of amoxicillin in porcine tissues by UPLC-MS/MS with internal standard".JOURNAL OF FOOD COMPOSITION AND ANALYSIS 92(2020).