干旱区生态环境知识资源中心(Arid): Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae

Arid

DOI	10.1073/pnas.1906904117
	Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae
	Peredo, Elena L.; Cardon, Zoe G.
通讯作者	Peredo, EL
来源期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
出版年	2020
卷号	117 期号:29 页码:17438-17445
英文摘要	Among green plants, desiccation tolerance is common in seeds and spores but rare in leaves and other vegetative green tissues. Over the last two decades, genes have been identified whose expression is induced by desiccation in diverse, desiccation-tolerant (DT) taxa, including, e.g., late embryogenesis abundant proteins (LEA) and reactive oxygen species scavengers. This up-regulation is observed in DT resurrection plants, mosses, and green algae most closely related to these Embryophytes. Here we test whether this same suite of protective genes is up-regulated during desiccation in even more distantly related DT green algae, and, importantly, whether that up-regulation is unique to DT algae or also occurs in a desiccation-intolerant relative. We used three closely related aquatic and desert-derived green microalgae in the family Scenedesmaceae and capitalized on extraordinary desiccation tolerance in two of the species, contrasting with desiccation intolerance in the third. We found that during desiccation, all three species increased expression of common protective genes. The feature distinguishing gene expression in DT algae, however, was extensive down-regulation of gene expression associated with diverse metabolic processes during the desiccation time course, suggesting a switch from active growth to energy-saving metabolism. This widespread downshift did not occur in the desiccation-intolerant taxon. These results show that desiccation-induced up-regulation of expression of protective genes may be necessary but is not sufficient to confer desiccation tolerance. The data also suggest that desiccation tolerance may require induced protective mechanisms operating in concert with massive down-regulation of gene expression controlling numerous other aspects of metabolism.
英文关键词	aquatic green algae desert-evolved green algae extremophiles microbiotic crusts Scenedesmaceae
类型	Article
语种	英语
开放获取类型	Green Published, Other Gold
收录类别	SCI-E
WOS记录号	WOS:000554723600016
WOS关键词	RNA-SEQ ; PROTEINS ; RECOVERY ; TRANSCRIPTOMICS ; ARCHITECTURE ; CHLOROPHYTA ; ORGANISMS ; CURVATURE ; EVOLUTION ; PLANTS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325340
作者单位	[Peredo, Elena L.; Cardon, Zoe G.] Marine Biol Lab, Ecosyst Ctr, Woods Hole, MA 02543 USA
推荐引用方式 GB/T 7714	Peredo, Elena L.,Cardon, Zoe G.. Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae[J],2020,117(29):17438-17445.
APA	Peredo, Elena L.,&Cardon, Zoe G..(2020).Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(29),17438-17445.
MLA	Peredo, Elena L.,et al."Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.29(2020):17438-17445.