干旱区生态环境知识资源中心(Arid): Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses

Arid

DOI	10.1016/j.sajb.2020.06.011
	Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses
	Shinde, Harshraj; Dudhate, Ambika; Anand, Lakshay; Tsugama, Daisuke; Gupta, Shashi K.; Liu, Shenkui; Takano, Tetsuo
通讯作者	Takano, T
来源期刊	SOUTH AFRICAN JOURNAL OF BOTANY
ISSN	0254-6299
EISSN	1727-9321
出版年	2020
卷号	132 页码:395-402
英文摘要	Pearl millet [Pennisetum glaucum (L.) R. Br.] is an important crop in arid and semi-arid areas of India and Africa. It is well known for its tolerance to abiotic stresses, but it lags behind other cereals in terms of research and development. MicroRNAs (miRNAs) are a versatile group of small regulatory RNAs of 20-22 bases that play important roles in plant growth, development, and stress responses. However, the regulatory mechanisms underlying miRNA-mediated responses to salinity stress in pearl millet are still unclear. In this study, we performed small RNA sequencing to identify conserved and novel miRNAs from the salinity tolerant pearl millet genotype. In total, 130 million sequence reads were generated, and 81 conserved and 14 novel miRNAs were identified as salinity stress responsive microRNAs. We also performed target prediction for these miRNAs, and a total of 448 pearl millet mRNAs were identified as the targets. Among these target mRNAs, 122 (similar to 25%) encode transcription factors. A pathway analysis showed that differentially expressed miRNAs and their target genes can regulate the auxin response pathway. Quantitative real-time PCR analysis of miRNAs and their targets showed consistent expression patterns. These results suggest that miRNAs play a role in salinity stress tolerance in pearl millet. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
英文关键词	Pearl millet Salinity stress miRNAs Transcription factors Auxin response pathway
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000554672800007
WOS关键词	WIDE IDENTIFICATION ; PENNISETUM-GLAUCUM ; CLONING
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	International Crops Research Institute for the Semi-Arid Tropics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325336
作者单位	[Shinde, Harshraj; Dudhate, Ambika; Tsugama, Daisuke; Takano, Tetsuo] Univ Tokyo, Asian Nat Environm Sci Ctr, Nishitokyo, Tokyo 1880002, Japan; [Shinde, Harshraj; Anand, Lakshay] Univ Kentucky, Environm Epigenet & Genet Grp, Dept Hort, Coll Agr Food & Environm, Lexington, KY USA; [Gupta, Shashi K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Telangana, India; [Liu, Shenkui] Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Shinde, Harshraj,Dudhate, Ambika,Anand, Lakshay,et al. Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses[J]. International Crops Research Institute for the Semi-Arid Tropics,2020,132:395-402.
APA	Shinde, Harshraj.,Dudhate, Ambika.,Anand, Lakshay.,Tsugama, Daisuke.,Gupta, Shashi K..,...&Takano, Tetsuo.(2020).Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses.SOUTH AFRICAN JOURNAL OF BOTANY,132,395-402.
MLA	Shinde, Harshraj,et al."Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses".SOUTH AFRICAN JOURNAL OF BOTANY 132(2020):395-402.