干旱区生态环境知识资源中心(Arid): New MiSeq based strategy exposed plant-preferential arbuscular mycorrhizal fungal communities in arid soils of Mexico

Arid

DOI	10.1007/s13199-020-00698-5
	New MiSeq based strategy exposed plant-preferential arbuscular mycorrhizal fungal communities in arid soils of Mexico
	Senes-Guerrero, Carolina; Gimenez, Salvador; Pacheco, Adriana; Gradilla-Hernandez, Misael Sebastian; Schuessler, Arthur
通讯作者	Senes-Guerrero, C
来源期刊	SYMBIOSIS
ISSN	0334-5114
EISSN	1878-7665
出版年	2020
卷号	81 期号:3 页码:235-246
英文摘要	Arbuscular mycorrhizal fungi (AMF) are obligate symbionts ofc. 80% of land plants, having enormous ecological and economic impact, as they often improve crop plant nutrition and yield. DNA-based identification with molecular markers is used to analyze AM fungal communities in the field, but reaching species level taxonomic resolution remains challenging. Thus, currently there is no consensus on how to analyze high-throughput sequences and assign them into species. Here, a new sequencing strategy combined with taxonomic affiliations implemented with an evolutionary placement algorithm (EPA) was established. It is based on sequencing ac. 450 bp region of the large subunit (LSU) ribosomal rRNA gene with the MiSeq-Illumina platform. The method is suitable for the discrimination of closely related AMF species and was used to study host-AMF preferences in roots of Pequin pepper, soybean and orange at one location in the arid northeast of Mexico. Twenty AM fungal species from 13 genera were detected. Phylogenetic affiliation of reads to species revealed crop preferential associations. In Pequin pepper roots, severalRhizophagusspecies represented most of the community,Rhizophagus clarusbeing the most abundant. The soybean AM fungal community was dominated byRhizophagus irregularisandFunneliformis mosseaeand that of orange by several species ofDominikia,some of them only found in this crop. Unraveling the AMF-plant preferences of important crops by an affordable and robust sequencing method, combined with phylotaxonomic AMF species resolution, is an important tool to obtain taxonomic units that are meaningful in both biological and ecological studies.
英文关键词	Arbuscular mycorrhizal fungi DNA-based species monitoring Citrus Evolutionary placement algorithm Pepper Soybean
类型	Article
语种	英语
开放获取类型	Other Gold
收录类别	SCI-E
WOS记录号	WOS:000554450000001
WOS关键词	ROOTS ; L. ; DIVERSITY ; CITRUS ; FIELD ; TOLERANCE ; DYNAMICS ; READS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325325
作者单位	[Senes-Guerrero, Carolina; Gimenez, Salvador; Pacheco, Adriana] Tecnol Monterrey, Ctr Biotecnol FEMSA, Escuela Ingn & Ciencias, Ave Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico; [Senes-Guerrero, Carolina; Gradilla-Hernandez, Misael Sebastian] Tecnol Monterrey, Escuela Ingn & Ciencias, Ave Gen Ramon Corona 2514, Zapopan 45138, Jal, Mexico; [Schuessler, Arthur] SYMPLANTA GmbH & Co KG, Graupnerweg 42, D-64287 Darmstadt, Germany
推荐引用方式 GB/T 7714	Senes-Guerrero, Carolina,Gimenez, Salvador,Pacheco, Adriana,et al. New MiSeq based strategy exposed plant-preferential arbuscular mycorrhizal fungal communities in arid soils of Mexico[J],2020,81(3):235-246.
APA	Senes-Guerrero, Carolina,Gimenez, Salvador,Pacheco, Adriana,Gradilla-Hernandez, Misael Sebastian,&Schuessler, Arthur.(2020).New MiSeq based strategy exposed plant-preferential arbuscular mycorrhizal fungal communities in arid soils of Mexico.SYMBIOSIS,81(3),235-246.
MLA	Senes-Guerrero, Carolina,et al."New MiSeq based strategy exposed plant-preferential arbuscular mycorrhizal fungal communities in arid soils of Mexico".SYMBIOSIS 81.3(2020):235-246.