干旱区生态环境知识资源中心(Arid): Role of urinary cations in the etiology of interstitial cystitis: A multisite study

Arid

DOI	10.1111/iju.14293
	Role of urinary cations in the etiology of interstitial cystitis: A multisite study
	Parsons, C. Lowell; Argade, Sulabha; Evans, Robert J.; Proctor, Jeffrey; Nickel, J. Curtis; Rosenberg, Matt T.; Bosch, Philip C.
通讯作者	Parsons, CL
来源期刊	INTERNATIONAL JOURNAL OF UROLOGY
ISSN	0919-8172
EISSN	1442-2042
出版年	2020
卷号	27 期号:9 页码:731-735
英文摘要	Objective To determine whether patients with interstitial cystitis have elevated levels of toxic urinary cations, to identify and quantify these cationic metabolites, and to assess their cytotoxicity. Methods Isolation of cationic fraction was achieved by solid phase extraction using an Oasis MCX cartridge on urine specimens from interstitial cystitis patients and controls. C(18)reverse phase high-performance liquid chromatography was used to profile cationic metabolites, and they were quantified by the area under the peaks and normalized to creatinine. Major cationic fraction peaks were identified by reverse phase high-performance liquid chromatography and liquid chromatography-mass spectrometry. HTB-4 urothelial cells were used to determine the cytotoxicity of cationic fraction and of individual metabolites. Results The reverse phase high-performance liquid chromatography analysis was carried out on cationic fraction metabolites isolated from urine samples of 70 interstitial cystitis patients and 34 controls. The mean for controls versus patients was 3.84 (standard error of the mean 0.20) versus 6.71 (0.37) mAU*min/mu g creatinine, respectively (P = 0.0001). The cationic fraction cytotoxicity normalized to creatinine for controls versus patients in mean percentage was -7.79% (standard error of the mean 3.32%) versus 20.03% (standard error of the mean 2.75%;P < 0.0005). The major toxic cations were 1-methyladenosine, 1-methylguanine, N-2,N-2-dimethylguanosine and L-tryptophan. Conclusions These data confirm significant elevation of toxic cations in the urine of interstitial cystitis patients. These toxic cations likely represent a primary cause of interstitial cystitis, as they can injure the bladder mucus and initiate an epithelial leak.
英文关键词	cytotoxicity interstitial cystitis liquid chromatography-mass spectrometry urinary cations urinary metabolites
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000552806400001
WOS关键词	TAMM-HORSFALL PROTEIN ; BLADDER SURFACE GLYCOSAMINOGLYCANS ; CYTOPROTECTIVE ACTIVITY ; INTRAVESICAL POTASSIUM ; PERMEABILITY ; MARKERS ; SYMPTOMS
WOS类目	Urology & Nephrology
WOS研究方向	Urology & Nephrology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/325236
作者单位	[Parsons, C. Lowell; Argade, Sulabha; Bosch, Philip C.] Univ Calif San Diego, Dept Urol, 200 W Arbor Dr, La Jolla, CA 92103 USA; [Evans, Robert J.] Wake Forest Baptist Med Ctr, Dept Urol, Winston Salem, NC USA; [Proctor, Jeffrey] Georgia Urol, Atlanta, GA USA; [Nickel, J. Curtis] Queens Univ, Sch Med, Kingston, ON, Canada; [Rosenberg, Matt T.] Mid Michigan Hlth Ctr, Jackson, MI USA
推荐引用方式 GB/T 7714	Parsons, C. Lowell,Argade, Sulabha,Evans, Robert J.,et al. Role of urinary cations in the etiology of interstitial cystitis: A multisite study[J],2020,27(9):731-735.
APA	Parsons, C. Lowell.,Argade, Sulabha.,Evans, Robert J..,Proctor, Jeffrey.,Nickel, J. Curtis.,...&Bosch, Philip C..(2020).Role of urinary cations in the etiology of interstitial cystitis: A multisite study.INTERNATIONAL JOURNAL OF UROLOGY,27(9),731-735.
MLA	Parsons, C. Lowell,et al."Role of urinary cations in the etiology of interstitial cystitis: A multisite study".INTERNATIONAL JOURNAL OF UROLOGY 27.9(2020):731-735.