干旱区生态环境知识资源中心(Arid): A Fog-Irrigated Soil Substrate System Unifies and Optimizes Cyanobacterial Biocrust Inoculum Production

Arid

DOI	10.1128/AEM.00624-20
	A Fog-Irrigated Soil Substrate System Unifies and Optimizes Cyanobacterial Biocrust Inoculum Production
	Nelson, Corey; Giraldo-Silva, Ana; Garcia-Pichel, Ferran
通讯作者	Garcia-Pichel, F
来源期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
EISSN	1098-5336
出版年	2020
卷号	86 期号:13
英文摘要	Biological soil crusts (biocrusts) are globally important microbial communities inhabiting the top layer of soils. They provide multiple services to dryland ecosystems but are particularly vulnerable to anthropogenic disturbance from which they naturally recover only slowly. Assisted inoculation with cyanobacteria is held as a promising approach to promote biocrust regeneration. Two different methodologies have been developed for this purpose: mass cultivation of biocrust pioneer species (such as the cyanobacteria Microcoleus spp.) on cellulose supports, and polymicrobial cultivation of biocrusts in soils within greenhouse settings. Here, we aimed to test a novel method to grow cyanobacterial biocrust inoculum based on fog irrigation of soil substrates (FISS) that can be used with either culture-based or mixed-community approaches. We found that the FISS system presents clear advantages over previous inoculum production methodologies; overall, FISS eliminates the need for specialized facilities and decreases user effort. Specifically, there were increased microbial yields and simplification of design compared to those of the culture-based and mixed-community approaches, respectively. Its testing also allows us to make recommendations on underexplored aspects of biocrust restoration: (i) field inoculation levels should be equal to or greater than the biomass found in the substrate and (ii) practices regarding evaluation of cyanobacterial biomass should, under certain circumstances, include proxies additional to chlorophyll a. IMPORTANCE Biocrust inoculum production for use in dryland rehabilitation is a powerful tool in combating the degradation of dryland ecosystems. However, the facilities and effort required to produce high-quality inoculum are often a barrier to effective large-scale implementation by land managers. By unifying and optimizing the two foremost methods for cyanobacterial biocrust inoculum production, our work improves on the ease and cost with which biocrust restoration technology can be translated to practical widespread implementation.
英文关键词	biocrusts biological soil crusts cyanobacteria cultivation soil restoration degraded dryland soils
类型	Article
语种	英语
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000546650600022
WOS关键词	COLORADO PLATEAU ; DESERT CRUSTS ; TEMPERATURE ; DIVERSITY ; RECOVERY ; COMMUNITY ; CULTURE ; LIGHT ; CULTIVATION ; DISTURBANCE
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
来源机构	Arizona State University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324974
作者单位	[Nelson, Corey; Giraldo-Silva, Ana; Garcia-Pichel, Ferran] Arizona State Univ, Ctr Fundamental & Appl Microbi, Biodesign Inst, Tempe, AZ 85281 USA; [Nelson, Corey; Giraldo-Silva, Ana; Garcia-Pichel, Ferran] Arizona State Univ, Sch Life Sci, Tempe, AZ 85281 USA
推荐引用方式 GB/T 7714	Nelson, Corey,Giraldo-Silva, Ana,Garcia-Pichel, Ferran. A Fog-Irrigated Soil Substrate System Unifies and Optimizes Cyanobacterial Biocrust Inoculum Production[J]. Arizona State University,2020,86(13).
APA	Nelson, Corey,Giraldo-Silva, Ana,&Garcia-Pichel, Ferran.(2020).A Fog-Irrigated Soil Substrate System Unifies and Optimizes Cyanobacterial Biocrust Inoculum Production.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,86(13).
MLA	Nelson, Corey,et al."A Fog-Irrigated Soil Substrate System Unifies and Optimizes Cyanobacterial Biocrust Inoculum Production".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 86.13(2020).