干旱区生态环境知识资源中心(Arid): Standardization of 1-and 2-D protein profiling of Haloxylon species: Basis of proteome analysis of haloxerophytes

Arid

	Standardization of 1-and 2-D protein profiling of Haloxylon species: Basis of proteome analysis of haloxerophytes
	Goswami, Deepmala; Dagla, Harchand R.
通讯作者	Dagla, HR
来源期刊	INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY
ISSN	0019-5189
EISSN	0975-1009
出版年	2020
卷号	58 期号:7 页码:487-492
英文摘要	One and two dimensional electrophoresis is a powerful technique for separation of proteins from complex biological sources. These are effective techniques of gene expression analysis at translational level especially for the species of which genome is not yet sequenced. High resolution electrophoresis separation of proteins from complex biological systems is crucial in protein science. Three protein extraction methods, such as Phenol, Multi detergent and TCA acetone were analyzed in the present investigation to find out appropriate protein extraction method amenable to one dimensional (1-D) and two dimensional (2-D) protein profiling of Haloxylon recurvum and H. salicornicum. Plant species selected for the present investigation are major components of saline and arid ecosystem and valuable source of food additives, nutritional ingredients, herbal medicines and fuel in the region. Phenolic components present in these plants are major hindrances for extraction of pure proteins. High concentrations of good quality proteins represented by clear, intense and more bands/spots in 1-D and 2-D gels were extracted with Phenol extraction method in comparison to TCA acetone and Multi detergent methods. This is possibly the first report on extraction and purification of proteins amenable to 1-D and 2-D proteome profiling of Haloxylon species of Indian arid zone, and forms a basis for further comparative proteome analysis of these two contrasting species with respect to salinity and drought tolerant ability for prospects.
英文关键词	Food additives Khar Phenolic components Protein extraction Proteome analysis
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000546329700006
WOS关键词	RECALCITRANT PLANT ; 2-DIMENSIONAL ELECTROPHORESIS ; GEL-ELECTROPHORESIS ; EXTRACELLULAR-MATRIX ; EXTRACTION METHODS ; APOPLAST PROTEOME ; L. ; TISSUES ; SALT ; LEAF
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324957
作者单位	[Goswami, Deepmala; Dagla, Harchand R.] Jai Narain Vyas Univ, Dept Bot, Plant Biotechnol & Mol Biol Lab, Jodhpur, Rajasthan, India
推荐引用方式 GB/T 7714	Goswami, Deepmala,Dagla, Harchand R.. Standardization of 1-and 2-D protein profiling of Haloxylon species: Basis of proteome analysis of haloxerophytes[J],2020,58(7):487-492.
APA	Goswami, Deepmala,&Dagla, Harchand R..(2020).Standardization of 1-and 2-D protein profiling of Haloxylon species: Basis of proteome analysis of haloxerophytes.INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY,58(7),487-492.
MLA	Goswami, Deepmala,et al."Standardization of 1-and 2-D protein profiling of Haloxylon species: Basis of proteome analysis of haloxerophytes".INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY 58.7(2020):487-492.