干旱区生态环境知识资源中心(Arid): In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert

Arid

DOI	10.1007/s11240-020-01876-5
	In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert
	Choudhary, Dheeraj; Rai, Manoj K.; Shekhawat, N. S.; Kataria, Vinod
通讯作者	Kataria, V
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
EISSN	1573-5044
出版年	2020
卷号	142 期号:3 页码:519-526
英文摘要	Farsetia macrantha Blatt. & Hallb. is an endemic and critically endangered plant species of Indian Thar desert. Being an endemic and threatened plant species, there is need to develop an efficient in vitro regeneration protocol for its conservation. Since, there is no published report on in vitro regeneration of F. macrantha, therefore, present study was designed to develop an efficient micropropagation method of E macrantha along with foliar micromorphological studies. Cotyledonary node taken from 15 to 20 days old seedlings was used as an explant for in vitro regeneration. MS medium supplemented with additives ascorbic acid (50 mg l (-1)), citric acid (25 mg l (-1)), L-arginine (25 mg l (-1)) and adenine sulphate (25 mg l (-1)) and 0.5 mg l (-1) BA was best for shoot bud induction. Different types, concentrations and combinations of cytokinins (BA, Kin and mT) and auxin (IAA) tested for shoot multiplication, maximum number of shoots (25.10 +/- 0.69) regenerated on MS medium containing additives and combination of two cytokinins 0.5 mg l (-1) BA + 0.25 mg l (-1) Kin. Microshoots were rooted successfully with highest rooting response (75.59%) on 1/2 MS medium containing 2.0 mg l (-1) IBA. In vitro rooted plants were hardened with- 75% survival rate. Comparative foliar micromorphological studies conducted among in vitro grown and hardened plants suggest the developmental changes of micropropagated plants during acclimatization. The present in vitro regeneration protocol could be used for large-scale propagation and conservation of E. macrantha.
英文关键词	Acclimatization Endemic and endangered plant Foliar micromorphology Micropropagation Plant growth regulators
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000545075200001
WOS关键词	SHOOT REGENERATION ; META-TOPOLIN ; IMPROVED MICROPROPAGATION ; GENETIC FIDELITY ; SOMATIC EMBRYOGENESIS ; VAR. CAPITATA ; L. ; COTYLEDON ; CABBAGE ; ORGANOGENESIS
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324917
作者单位	[Choudhary, Dheeraj; Shekhawat, N. S.; Kataria, Vinod] Jai Narain Vyas Univ, Biotechnol Unit, Dept Bot, UGC Ctr Adv Study, New Campus, Jodhpur 342001, Rajasthan, India; [Rai, Manoj K.] Indira Gandhi Natl Tribal Univ, Dept Environm Sci, Amarkantak 484887, Madhya Pradesh, India
推荐引用方式 GB/T 7714	Choudhary, Dheeraj,Rai, Manoj K.,Shekhawat, N. S.,et al. In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert[J],2020,142(3):519-526.
APA	Choudhary, Dheeraj,Rai, Manoj K.,Shekhawat, N. S.,&Kataria, Vinod.(2020).In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert.PLANT CELL TISSUE AND ORGAN CULTURE,142(3),519-526.
MLA	Choudhary, Dheeraj,et al."In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert".PLANT CELL TISSUE AND ORGAN CULTURE 142.3(2020):519-526.