干旱区生态环境知识资源中心(Arid): The aggregation pheromone phenylacetonitrile: Joint action with the entomopathogenic fungus Metarhizium anisopliae( )var. acridum and physiological and transcriptomic effects on Schistocerca gregaria nymphs

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DOI	10.1016/j.pestbp.2020.104594
	The aggregation pheromone phenylacetonitrile: Joint action with the entomopathogenic fungus Metarhizium anisopliae( )var. acridum and physiological and transcriptomic effects on Schistocerca gregaria nymphs
	Abdellaoui, Khemais; Miladi, Meriam; Mkhinini, Marouane; Boughattas, Iteb; Ben Hamouda, Amel; Hajji-Hedfi, Lobna; Tlili, Haithem; Acheuk, Fatma
通讯作者	Abdellaoui, K
来源期刊	PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
ISSN	0048-3575
EISSN	1095-9939
出版年	2020
卷号	167
英文摘要	The combined use of entomopathogenic fungi and sublethal rate of chemical insecticides or other biological control agents have been proposed as an environmentally and sustainable strategy in the control of locust pests. In this paper, the quarter and the half of the recommended dose of Metarhizium anisopliae var. acridum (1/4 and 1/2 Ma) and the aggregation pheromone (Phenylacetonitrile: PAN) were applied simultaneously and sequentially to Schistocerca gregaria fifth-instar nymphs. In addition, the physiological effects of PAN on locusts were assessed at the behavior, immune response, and biochemical level by evaluating for glutathione-S-transferase (GST), acetylcholinesterase inhibition (AChE), and malondialdehyde accumulation (MDA). Results showed that simultaneous application of PAN and the entomopathogenic fungus exhibited additive interaction. Synergistic interaction was also demonstrated when nymphs were exposed to PAN first, then treated with M. anisopliae var. acridum. Behavioral bioassay revealed that fifth-instar nymphs avoided the PAN odour and tended to remain away from the stimulus cup. In the choice assay, the pheromone significantly repelled the locusts at 2, 4, and 6 h of exposure which selected the PAN-free arena chamber. Moreover, treated nymphs become hyperactive and disoriented as evidenced by the cumulative distance travelled and the trajectory of locusts during the experiment. Immunological studies showed that PAN significantly decreased the differential haemocyte counts (prohemocytes and plasmatocytes) with a dose-response relationship. Data of biochemical analyzes showed that the PAN exposure reduced the activity of acetylcholinesterase and induced significantly the glutathione S-transferases and MDA concentration in the desert locust fifth-instar nymphs. Moreover, transcriptomic responses to the PAN exposure were evaluated using gene expression levels of CYP540 and GST. The transcript levels showed an up-regulation in GST expression level particularly in nymphs exposed for 4 and 6 h. A significant increase in CYP450 transcript level was also observed after 2 h of exposure, which decreased significantly after 4 and 6 h.
英文关键词	S. gregaria Phenylacetonitrile Metarhizium Physiology Oxidative stress Gene expression
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000540265900021
WOS关键词	DESERT LOCUST ; BEAUVERIA-BASSIANA ; MIGRATORY LOCUST ; IMMUNE FUNCTION ; DNA-DAMAGE ; INSECT ; PESTICIDES ; EXPRESSION ; ACID ; TRANSFERASES
WOS类目	Biochemistry & Molecular Biology ; Entomology ; Physiology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology ; Physiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324750
作者单位	[Abdellaoui, Khemais; Miladi, Meriam; Ben Hamouda, Amel; Hajji-Hedfi, Lobna] Sousse Univ, Higher Agron Inst Chott Mariem, Dept Biol Sci & Plant Protect, Sousse, Tunisia; [Mkhinini, Marouane; Boughattas, Iteb] Sousse Univ, Higher Agron Inst Chott Mariem, Lab Biochem & Environm Toxicol, Sousse, Tunisia; [Tlili, Haithem] Univ Tunis El Manar, Fac Sci Tunis, Dept Biol Sci, Tunis, Tunisia; [Acheuk, Fatma] Univ Boumerdes, Fac Sci, Lab Valorizat & Conservat Biol Resources Valcore, Dept Biol, Boumerdes, Algeria
推荐引用方式 GB/T 7714	Abdellaoui, Khemais,Miladi, Meriam,Mkhinini, Marouane,et al. The aggregation pheromone phenylacetonitrile: Joint action with the entomopathogenic fungus Metarhizium anisopliae( )var. acridum and physiological and transcriptomic effects on Schistocerca gregaria nymphs[J],2020,167.
APA	Abdellaoui, Khemais.,Miladi, Meriam.,Mkhinini, Marouane.,Boughattas, Iteb.,Ben Hamouda, Amel.,...&Acheuk, Fatma.(2020).The aggregation pheromone phenylacetonitrile: Joint action with the entomopathogenic fungus Metarhizium anisopliae( )var. acridum and physiological and transcriptomic effects on Schistocerca gregaria nymphs.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,167.
MLA	Abdellaoui, Khemais,et al."The aggregation pheromone phenylacetonitrile: Joint action with the entomopathogenic fungus Metarhizium anisopliae( )var. acridum and physiological and transcriptomic effects on Schistocerca gregaria nymphs".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 167(2020).