干旱区生态环境知识资源中心(Arid): DiverseThaumarchaeotaDominate Subsurface Ammonia-oxidizing Communities in Semi-arid Floodplains in the Western United States

Arid

DOI	10.1007/s00248-020-01534-5
	DiverseThaumarchaeotaDominate Subsurface Ammonia-oxidizing Communities in Semi-arid Floodplains in the Western United States
	Cardarelli, Emily L.; Bargar, John R.; Francis, Christopher A.
通讯作者	Francis, CA
来源期刊	MICROBIAL ECOLOGY
ISSN	0095-3628
EISSN	1432-184X
出版年	2020
卷号	80 期号:4 页码:778-792
英文摘要	Subsurface microbial communities mediate biogeochemical transformations that drive both local and ecosystem-level cycling of essential elements, including nitrogen. However, their study has been largely limited to the deep ocean, terrestrial mines, caves, and topsoils (< 30 cm). Here, we present regional insights into the microbial ecology of aerobic ammonia oxidation within the terrestrial subsurface of five semi-arid riparian sites spanning a 900-km N-S transect. We sampled sediments, profiled communities to depths of <= 10 m, and compared them to reveal trends regionally within and surrounding the Upper Colorado River Basin (CRB). The diversity and abundance of ammonia-oxidizing microbial communities were evaluated in the context of subsurface geochemistry by applying a combination ofamoA(encoding ammonia monooxygenase subunit A) gene sequencing, quantitative PCR, and geochemical techniques. Analysis of 898amoAsequences from ammonia-oxidizing archaea (AOA) and bacteria (AOB) revealed extensive ecosystem-scale diversity, including archaealamoAsequences from four of the five major AOA lineages currently found worldwide as well as distinct AOA ecotypes associated with naturally reduced zones (NRZs) and hydrogeochemical zones (unsaturated, capillary fringe, and saturated). Overall, AOA outnumber AOB by 2- to 5000-fold over this regional scale, suggesting that AOA may play a prominent biogeochemical role in nitrification within terrestrial subsurface sediments.
英文关键词	Terrestrial subsurface amoA Ammonia-oxidizing archaea Ammonia-oxidizing bacteria Nitrification Thaumarchaeota
类型	Article
语种	英语
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000539949900001
WOS关键词	MICROBIAL COMMUNITIES ; NICHE SPECIALIZATION ; SPATIAL-DISTRIBUTION ; ARCHAEA ; DIVERSITY ; BACTERIA ; SOIL ; SEDIMENTS ; NITRIFICATION ; ABUNDANCE
WOS类目	Ecology ; Marine & Freshwater Biology ; Microbiology
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324726
作者单位	[Cardarelli, Emily L.; Francis, Christopher A.] Stanford Univ, Dept Earth Syst Sci, Stanford, CA 94305 USA; [Bargar, John R.] SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USA
推荐引用方式 GB/T 7714	Cardarelli, Emily L.,Bargar, John R.,Francis, Christopher A.. DiverseThaumarchaeotaDominate Subsurface Ammonia-oxidizing Communities in Semi-arid Floodplains in the Western United States[J],2020,80(4):778-792.
APA	Cardarelli, Emily L.,Bargar, John R.,&Francis, Christopher A..(2020).DiverseThaumarchaeotaDominate Subsurface Ammonia-oxidizing Communities in Semi-arid Floodplains in the Western United States.MICROBIAL ECOLOGY,80(4),778-792.
MLA	Cardarelli, Emily L.,et al."DiverseThaumarchaeotaDominate Subsurface Ammonia-oxidizing Communities in Semi-arid Floodplains in the Western United States".MICROBIAL ECOLOGY 80.4(2020):778-792.