干旱区生态环境知识资源中心(Arid): Molecular identification of zoonotic hookworms in dogs from four counties of Kenya

Arid

DOI	10.1017/S0022149X1900018X
	Molecular identification of zoonotic hookworms in dogs from four counties of Kenya
	Mulinge, E.; Njenga, S. M.; Odongo, D.; Magambo, J.; Zeyhle, E.; Mbae, C.; Kagendo, D.; Kanyi, H.; Traub, R. J.; Wassermann, M.; Kern, P.; Romig, T.
通讯作者	Mulinge, E
来源期刊	JOURNAL OF HELMINTHOLOGY
ISSN	0022-149X
EISSN	1475-2697
出版年	2020
卷号	94
英文摘要	All canine hookworms are known to be zoonotic, causing infections ranging from transient skin irritations to prolonged 'creeping eruptions', eosinophilic enteritis and even patent intestinal infections. There is little information on canine hookworm species and their public health significance in sub-Saharan Africa. This study determined the prevalence and species of hookworms in dogs from different climatic zones of Kenya. Dog faecal samples were collected from the environment, and hookworm eggs were isolated by zinc chloride flotation and subjected to DNA extraction. Polymerase chain reaction (PCR) assays targeting the internal transcribed spacer (ITS) 1 and 2, 5.8S and 28S ribosomal RNA of Ancylostoma spp. and Uncinaria stenocephala were performed, and hookworm species were identified by PCR-restriction fragment length polymorphism (RFLP) or DNA sequencing. Hookworm eggs were detected by microscopy in 490/1621 (30.23%, 95% CI 28.01-32.54) faecal samples. Estimates of faecal prevalence were high in counties receiving higher rainfall (Narok 46.80%, Meru 44.88%) and low in those with a more arid climate (Isiolo 19.73%, Turkana 11.83%). In a subset of 70 faecal samples, Ancylostoma caninum (n = 59) was the most common species, followed by A. braziliense (n = 10) and A. cf. duodenale (n = 1). This study reports for the first time the detection of A. cf. duodenale in dog faeces and zoonotic hookworm species in Kenyan dogs. These findings emphasize the need for control measures such as enforcing laws for restraining stray dogs, regular deworming of dogs, and public health awareness programmes aimed at informing communities on outdoor use of footwear.
英文关键词	Ancylostoma braziliense Ancylostoma caninum Ancylostoma duodenale dogs hookworm species Kenya
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000538812200001
WOS关键词	CUTANEOUS LARVA MIGRANS ; SAO-PAULO STATE ; ANCYLOSTOMA-CEYLANICUM ; ECHINOCOCCUS-MULTILOCULARIS ; GASTROINTESTINAL PARASITES ; NECATOR-AMERICANUS ; PCR-RFLP ; INFECTION ; COMMUNITY ; CATS
WOS类目	Parasitology ; Zoology
WOS研究方向	Parasitology ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324672
作者单位	[Mulinge, E.; Njenga, S. M.; Mbae, C.; Kanyi, H.] Kenya Govt Med Res Ctr, Nairobi, Kenya; [Mulinge, E.; Odongo, D.] Univ Nairobi, Sch Biol Sci, Nairobi, Kenya; [Magambo, J.; Zeyhle, E.; Kagendo, D.] Meru Univ Sci & Technol, Meru, Kenya; [Traub, R. J.] Univ Melbourne, Fac Vet & Agr Sci, Parkville, Vic, Australia; [Wassermann, M.; Romig, T.] Univ Hohenheim, Parasitol Unit, Stuttgart, Germany; [Kern, P.] Univ Hosp Ulm, Dept Med 3, Ulm, Germany
推荐引用方式 GB/T 7714	Mulinge, E.,Njenga, S. M.,Odongo, D.,et al. Molecular identification of zoonotic hookworms in dogs from four counties of Kenya[J],2020,94.
APA	Mulinge, E..,Njenga, S. M..,Odongo, D..,Magambo, J..,Zeyhle, E..,...&Romig, T..(2020).Molecular identification of zoonotic hookworms in dogs from four counties of Kenya.JOURNAL OF HELMINTHOLOGY,94.
MLA	Mulinge, E.,et al."Molecular identification of zoonotic hookworms in dogs from four counties of Kenya".JOURNAL OF HELMINTHOLOGY 94(2020).