干旱区生态环境知识资源中心(Arid): A coupled method of on-line solid phase extraction with the UHPLC- MS/MS for detection of sulfonamides antibiotics residues in aquaculture

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DOI	10.1016/j.chemosphere.2020.126765
	A coupled method of on-line solid phase extraction with the UHPLC- MS/MS for detection of sulfonamides antibiotics residues in aquaculture
	Li, Tao; Wang, Ce; Xu, Zhaoan; Chakraborty, Amit
通讯作者	Wang, C
来源期刊	CHEMOSPHERE
ISSN	0045-6535
EISSN	1879-1298
出版年	2020
卷号	254
英文摘要	The use of a variety of antibiotics in fish farming raises serious concern about the development of antibiotic resistance. Sulfonamides antibiotics (SAs), which are widely used in aquaculture and generate large eco-toxicological effects with significant mutagenicity and teratogenic consequences, are still difficult to determine in aquatic organisms. In this study, an automatic technology was developed by coupling on-line solid phase extraction system (on-line SPE) with ultra-high-performance liquid chromatography spectrometry-mass spectrometry (UHPLC-MS/MS). Particularly, using a single on-line column in the process of sample pretreatment, e.g., HLB or C18, phospholipids that potentially caused the matrix effect cannot be removed form biological sample. We applied a mixed cation exchange column (Oasis (R) MCX) connected with a hydrophilic lipophilic balance column (Oasis (R) HLB) in series in on-line SPE clean-up to remove interferences and finally obtained a clear and stable eluant. The on-line SPE working conditions and UHPLC-MS/MS parameters were optimized for their sensitivity, accuracy, decision limit, and detection capability, which were further calibrated for fish, shrimp and crab. The results showed that the limits of detection and limits of quantification ranged from 1.46 to 15.5 ng/kg, and 4.90 -51.6 ng/kg, respectively. Accuracy values covered 71.5%-102% at the three concentration levels (0.1, 0.5, 1.0 mu g/kg) for all compounds and average repeatability (relative standard deviation, RSD%) ranged from 3.47% to 14.2%. This on-line SPE coupled with UHPLC-MS/MS method is a way forward for an automatic, powerful detection technology for determination of antibiotics from complex matrix. (C) 2020 Elsevier Ltd. All rights reserved.
英文关键词	on-line SPE Sulfonamides antibiotics Aquatic organisms UHPLC-MS/MS
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000538150400026
WOS关键词	TANDEM MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; MULTI-RESIDUE ; SPE-LC ; TRACE LEVELS ; WASTE-WATER ; FISH ; SAMPLES ; QUANTIFICATION ; METABOLITES
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324657
作者单位	[Li, Tao; Xu, Zhaoan] Monitoring Bur Hydrol & Water Resources Taihu Bas, Wuxi, Jiangsu, Peoples R China; [Wang, Ce] Southeast Univ, Sch Energy & Environm, Nanjing 210096, Peoples R China; [Wang, Ce] Southeast Univ, Key Lab Environm Med Engn, Minist Educ, Nanjing 210096, Peoples R China; [Chakraborty, Amit] Cent Univ Rajasthan, Sch Math Stat & Computat Sci, Ajmer, India
推荐引用方式 GB/T 7714	Li, Tao,Wang, Ce,Xu, Zhaoan,et al. A coupled method of on-line solid phase extraction with the UHPLC- MS/MS for detection of sulfonamides antibiotics residues in aquaculture[J],2020,254.
APA	Li, Tao,Wang, Ce,Xu, Zhaoan,&Chakraborty, Amit.(2020).A coupled method of on-line solid phase extraction with the UHPLC- MS/MS for detection of sulfonamides antibiotics residues in aquaculture.CHEMOSPHERE,254.
MLA	Li, Tao,et al."A coupled method of on-line solid phase extraction with the UHPLC- MS/MS for detection of sulfonamides antibiotics residues in aquaculture".CHEMOSPHERE 254(2020).