干旱区生态环境知识资源中心(Arid): Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells

Arid

DOI	10.1016/j.ibmb.2020.103377
	Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells
	Mingels, Lina; Wynant, Niels; Santos, Dulce; Peeters, Paulien; Gansemans, Yannick; Billen, Johan; Van Nieuwerburgh, Filip; Vanden Broeck, Jozef
通讯作者	Mingels, L
来源期刊	INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN	0965-1748
EISSN	1879-0240
出版年	2020
卷号	122
英文摘要	The potential utility of RNA interference (RNAi) to control insect pests and viral infections depends largely on the target organism's ability to systemically spread the RNAi response. The efficacy of systemic RNAi varies among insects, though it has been shown to be high in the red flour beetle, Tribolium castaneum. We identified an extracellular RNAi signal that is present in the culture medium of T. castaneum (TcA) cells after treatment with long dsRNA specific for a luciferase reporter gene. Luciferase-specific siRNAs were detected in extracellular vesicles (EVs) that were purified from the culture medium of these dsRNA-treated cells. Furthermore, by measuring the silencing of luciferase expression, we showed that these siRNA-containing EVs can act as an RNAi signal for recipient TcA cells. We have therefore shown that a systemic RNAi response upon dsRNA treatment can be effectively spread through EVs.
英文关键词	dsRNA Insect Intercellular communication RNAi siRNA Small RNA
类型	Article
语种	英语
开放获取类型	Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000537570800005
WOS关键词	DOUBLE-STRANDED-RNA ; RECEPTOR-MEDIATED ENDOCYTOSIS ; ANTIVIRAL DEFENSE ; DESERT LOCUST ; MESSENGER-RNA ; SYSTEMIC RNAI ; DSRNA ; INITIATION ; MICRORNAS ; SEQUENCES
WOS类目	Biochemistry & Molecular Biology ; Entomology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324621
作者单位	[Mingels, Lina; Wynant, Niels; Santos, Dulce; Peeters, Paulien; Vanden Broeck, Jozef] Katholieke Univ Leuven, Dept Biol, Mol Dev Physiol & Signal Transduct, B-3000 Leuven, Belgium; [Gansemans, Yannick; Van Nieuwerburgh, Filip] Univ Ghent, Lab Pharmaceut Biotechnol, B-9000 Ghent, Belgium; [Billen, Johan] Katholieke Univ Leuven, Dept Biol, Socioecol & Social Evolut, B-3000 Leuven, Belgium
推荐引用方式 GB/T 7714	Mingels, Lina,Wynant, Niels,Santos, Dulce,et al. Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells[J],2020,122.
APA	Mingels, Lina.,Wynant, Niels.,Santos, Dulce.,Peeters, Paulien.,Gansemans, Yannick.,...&Vanden Broeck, Jozef.(2020).Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,122.
MLA	Mingels, Lina,et al."Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 122(2020).