干旱区生态环境知识资源中心(Arid): Plant tissue succulence engineering improves water-use efficiency, water-deficit stress attenuation and salinity tolerance in Arabidopsis

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DOI	10.1111/tpj.14783
	Plant tissue succulence engineering improves water-use efficiency, water-deficit stress attenuation and salinity tolerance in Arabidopsis
	Lim, Sung Don; Mayer, Jesse A.; Yim, Won Cheol; Cushman, John C.
通讯作者	Cushman, JC
来源期刊	PLANT JOURNAL
ISSN	0960-7412
EISSN	1365-313X
出版年	2020
卷号	103 期号:3 页码:1049-1072
英文摘要	Tissue succulence (ratio of tissue water/leaf area or dry mass) or the ability to store water within living tissues is among the most successful adaptations to drought in the plant kingdom. This taxonomically widespread adaptation helps plants avoid the damaging effects of drought, and is often associated with the occupancy of epiphytic, epilithic, semi-arid and arid environments. Tissue succulence was engineered in Arabidopsis thaliana by overexpression of a codon-optimized helix-loop-helix transcription factor (VvCEB1(opt)) from wine grape involved in the cell expansion phase of berry development. VvCEB1(opt)-overexpressing lines displayed significant increases in cell size, succulence and decreased intercellular air space. VvCEB1(opt)-overexpressing lines showed increased instantaneous and integrated water-use efficiency (WUE) due to reduced stomatal conductance caused by reduced stomatal aperture and density resulting in increased attenuation of water-deficit stress. VvCEB1(opt)-overexpressing lines also showed increased salinity tolerance due to reduced salinity uptake and dilution of internal Na+ and Cl- as well as other ions. Alterations in transporter activities were further suggested by media and apoplastic acidification, hygromycin B tolerance and changes in relative transcript abundance patterns of various transporters with known functions in salinity tolerance. Engineered tissue succulence might provide an effective strategy for improving WUE, drought avoidance or attenuation, salinity tolerance, and for crassulacean acid metabolism biodesign.
英文关键词	basic helix-loop-helix transcription factor drought attenuation salinity tolerance rhizosphere acidification Arabidopsis thaliana
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000532958000001
WOS关键词	CRASSULACEAN ACID METABOLISM ; MEMBRANE H+-ATPASE ; DROUGHT TOLERANCE ; LEAF SUCCULENCE ; SALT TOLERANCE ; CLIMATE-CHANGE ; BIOENERGY PRODUCTION ; STOMATAL DENSITY ; K+ CHANNELS ; GLOBAL FOOD
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324452
作者单位	[Lim, Sung Don] Kangwon Natl Univ, Dept Appl Plant Sci, Chunchon 24341, South Korea; [Mayer, Jesse A.] Thermo Fisher Sci, Carlsbad, CA 92008 USA; [Yim, Won Cheol; Cushman, John C.] Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA
推荐引用方式 GB/T 7714	Lim, Sung Don,Mayer, Jesse A.,Yim, Won Cheol,et al. Plant tissue succulence engineering improves water-use efficiency, water-deficit stress attenuation and salinity tolerance in Arabidopsis[J],2020,103(3):1049-1072.
APA	Lim, Sung Don,Mayer, Jesse A.,Yim, Won Cheol,&Cushman, John C..(2020).Plant tissue succulence engineering improves water-use efficiency, water-deficit stress attenuation and salinity tolerance in Arabidopsis.PLANT JOURNAL,103(3),1049-1072.
MLA	Lim, Sung Don,et al."Plant tissue succulence engineering improves water-use efficiency, water-deficit stress attenuation and salinity tolerance in Arabidopsis".PLANT JOURNAL 103.3(2020):1049-1072.