干旱区生态环境知识资源中心(Arid): Functional relationships between aboveground and belowground spinach (Spinacia oleracea L., cv. Racoon) microbiomes impacted by salinity and drought

Arid

DOI	10.1016/j.scitotenv.2020.137207
	Functional relationships between aboveground and belowground spinach (Spinacia oleracea L., cv. Racoon) microbiomes impacted by salinity and drought
	Ibekwe, A. Mark; Ors, Selda; Ferreira, Jorge F. S.; Liu, Xuan; Suarez, Donald L.; Ma, Jincai; Ghasemimianaei, Alaleh; Yang, Ching-Hong
通讯作者	Ibekwe, AM
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2020
卷号	717
英文摘要	Salinity is a major problem facing agriculture in arid and semiarid regions of the world. This problem may vary among seasons affecting both above- and belowground plant microbiomes. However, very few studies have been conducted to examine the influence of salinity and drought on microbiomes and on their functional relationships. The objective for the study was to examine the effects of salinity and drought on above- and belowground spinach microbiomes and evaluate seasonal changes in their bacterial community composition and diversity. Furthermore, potential consequences for community functioning were assessed based on 16S V4 rRNA gene profiles by indirectly inferring the abundance of functional genes based on results obtained with Piphillin. The experiment was repeated three times from early fall to late spring in sand tanks planted with spinach (Spinacia oleracea L., cv. Racoon) grown with saline water of different concentrations and provided at different amounts. Proteobacteria, Cyanobacteria, and Bacteroidetes accounted for 77.1% of taxa detected in the rhizosphere; Proteobacteria, Bacteroidetes, and Actinobacteria accounted for 55.1% of taxa detected in soil, while Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria accounted for 55.35% of taxa detected in the phyllosphere. Salinity significantly affected root microbiome beta-diversity according to weighted abundances (p = 0.032) but had no significant effect on the relative abundances of microbial taxa (p = 0.568). Pathways and functional genes analysis of soil, rhizosphere, and phyllosphere showed that the most abundant functional genes were mapped to membrane transport, DNA repair and recombination, signal transduction, purine metabolism, translation-related protein processing, oxidative phosphorylation, bacterial motility protein secretion, and membrane receptor proteins. Monoterpenoid biosynthesis was the most significantly enriched pathway in rhizosphere samples when compared to the soil samples. Overall, the predictive abundances indicate that, functionally, the rhizosphere bacteria had the highest gene abundances and that salinity and drought affected the above- and belowground microbiomes differently. Published by Elsevier B.V.
英文关键词	Salinity Seasonal changes Piphillin Soil microbial communities Temporal variability Inferred pathways Genes
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000519994800056
WOS关键词	COMPATIBLE SOLUTE ; DIVERSITY ; TOLERANCE ; SEQUENCES ; BACTERIA ; ECOLOGY ; PLANTS
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/324311
作者单位	[Ibekwe, A. Mark; Ferreira, Jorge F. S.; Liu, Xuan; Suarez, Donald L.] USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA; [Ors, Selda] Ataturk Univ, Dept Agr Struct & Irrigat, TR-25240 Erzurum, Turkey; [Ma, Jincai] Jilin Univ, Key Lab Ground Water Resource & Environm, Minist Educ, Changchun 130021, Peoples R China; [Ma, Jincai] Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China; [Ghasemimianaei, Alaleh; Yang, Ching-Hong] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA
推荐引用方式 GB/T 7714	Ibekwe, A. Mark,Ors, Selda,Ferreira, Jorge F. S.,et al. Functional relationships between aboveground and belowground spinach (Spinacia oleracea L., cv. Racoon) microbiomes impacted by salinity and drought[J],2020,717.
APA	Ibekwe, A. Mark.,Ors, Selda.,Ferreira, Jorge F. S..,Liu, Xuan.,Suarez, Donald L..,...&Yang, Ching-Hong.(2020).Functional relationships between aboveground and belowground spinach (Spinacia oleracea L., cv. Racoon) microbiomes impacted by salinity and drought.SCIENCE OF THE TOTAL ENVIRONMENT,717.
MLA	Ibekwe, A. Mark,et al."Functional relationships between aboveground and belowground spinach (Spinacia oleracea L., cv. Racoon) microbiomes impacted by salinity and drought".SCIENCE OF THE TOTAL ENVIRONMENT 717(2020).