干旱区生态环境知识资源中心(Arid): Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix

Arid

DOI	10.3390/toxins12060374
	Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix
	Munoz-Solano, Borja; Gonzalez-Penas, Elena
通讯作者	Gonzalez-Penas, Elena
来源期刊	TOXINS
EISSN	2072-6651
出版年	2020
卷号	12 期号:6
英文摘要	Mycotoxins are toxic compounds for humans and animals that are produced by fungi. Mycotoxin contamination in feed is a global safety concern and effective control of these compounds in this matrix is needed. This study proposes a simple, cost-effective analytical method based on liquid chromatography coupled with a fluorescence detector, which is suitable for the routine monitoring of some of the most important mycotoxins in feed: aflatoxins (G2, G1, B2, and B1), zearalenone, and ochratoxins A and B. Mycotoxin extraction, chromatographic separation and quantification are carried out simultaneously for all mycotoxins. The extraction procedure is performed using acetonitrile, water and orthophosphoric acid (80:19:1). Purification of the extract is carried out using an OASIS PRIME HLB solid-phase extraction cartridge followed by a dispersive liquid-liquid microextraction procedure. Aflatoxins G1 and B1 are derivatized post-column (photochemical reactor at 254 nm) to increase their signal. The method has been validated in feed for pigs, cows, sheep, and poultry with very satisfactory results. The detection limits are 2 mu g/kg for aflatoxins B1 and G1, 0.64 mu g/kg for aflatoxins B2 and G2, 42 mu g/kg for zearalenone, and 5 mu g/kg for ochratoxins A and B. These values are low enough to allow for monitoring of these mycotoxins in feed. Global recovery values were between 73.6% and 88.0% for all toxins with a relative standard deviation (RSD) % < 7%. This methodology will facilitate laboratory control and analysis of mycotoxins in feed.
英文关键词	feed mycotoxins liquid chromatography fluorescence multidetection
类型	Article
语种	英语
国家	Spain
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000551188000062
WOS关键词	SOLID-PHASE EXTRACTION ; MULTI-MYCOTOXIN ; FUMONISIN B-1 ; ZEARALENONE ; HPLC ; INGREDIENTS ; PERFORMANCE ; VALIDATION ; DERIVATIZATION ; CONTAMINATION
WOS类目	Food Science & Technology ; Toxicology
WOS研究方向	Food Science & Technology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319818
作者单位	Univ Navarra, Sch Pharm & Nutr, Dept Pharmaceut Technol & Chem, Pamplona 31008, Spain
推荐引用方式 GB/T 7714	Munoz-Solano, Borja,Gonzalez-Penas, Elena. Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix[J],2020,12(6).
APA	Munoz-Solano, Borja,&Gonzalez-Penas, Elena.(2020).Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix.TOXINS,12(6).
MLA	Munoz-Solano, Borja,et al."Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix".TOXINS 12.6(2020).