干旱区生态环境知识资源中心(Arid): Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography-Tandem Mass Spectrometry Method

Arid

DOI	10.3390/toxins12060362
	Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography-Tandem Mass Spectrometry Method
	Panasiuk, Lukasz; Jedziniak, Piotr; Pietruszka, Katarzyna; Posyniak, Andrzej
通讯作者	Panasiuk, Lukasz
来源期刊	TOXINS
EISSN	2072-6651
出版年	2020
卷号	12 期号:6
英文摘要	A liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3Ac-DON), 15-acetyldeoxynivalenol (15Ac-DON), DON-3-glucoside (DON-3Glc) nivalenol and fusarenone-X in feedstuffs. Different techniques of sample preparation were tested: solid-liquid-extraction, QuEChERS, solid phase extraction with OASIS HLB columns or immunoaffinity columns and a Mycosep 225 Trich column. None of the six immunoaffinity columns tested showed cross-reactivity to all of the mycotoxins. Surprisingly, the results show that if the immunoaffinity columns bound 3Ac-DON, then they did not bind 15Ac-DON. The most efficient sample preparation was achieved with a Mycosep 225 Trich column clean-up. The chromatography was optimised to obtain full separation of all analytes (including 3Ac-DON and 15Ac-DON isomeric form). The validation results show the relative standard deviations for repeatability and reproducibility varied from 4% to 24%. The apparent recovery ranged between 92% and 97%, and the limit of quantification described a 1.30 to 50 mu g/kg range. The method trueness was satisfactory, as assessed by a proficiency test and analysis of reference material. A total of 99 feed samples were analysed by the developed method, revealing the presence of DON and DON-3Glc in 85% and 86% of examined animal feeds, respectively at concentrations between 1.70 and 1709 mu g/kg. The ratios DON-3Glc to DON in the surveyed feedstuffs were from a low of 3% to high of 59%.
英文关键词	type B trichothecenes modified mycotoxins isomer separation method validation
类型	Article
语种	英语
国家	Poland
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000551188000001
WOS关键词	LC-MS/MS METHOD ; IMMUNOAFFINITY COLUMNS ; ACETYLATED DERIVATIVES ; MASKED MYCOTOXINS ; CROSS-REACTIVITY ; FUSARIUM TOXINS ; ANIMAL FEED ; WHEAT ; CEREALS ; CLEANUP
WOS类目	Food Science & Technology ; Toxicology
WOS研究方向	Food Science & Technology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319817
作者单位	Natl Vet Res Inst, Dept Pharmacol & Toxicol, Al Partyzantow 57, PL-24100 Pulawy, Poland
推荐引用方式 GB/T 7714	Panasiuk, Lukasz,Jedziniak, Piotr,Pietruszka, Katarzyna,et al. Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography-Tandem Mass Spectrometry Method[J],2020,12(6).
APA	Panasiuk, Lukasz,Jedziniak, Piotr,Pietruszka, Katarzyna,&Posyniak, Andrzej.(2020).Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography-Tandem Mass Spectrometry Method.TOXINS,12(6).
MLA	Panasiuk, Lukasz,et al."Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography-Tandem Mass Spectrometry Method".TOXINS 12.6(2020).