干旱区生态环境知识资源中心(Arid): PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

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DOI	10.33073/pjm-2020-016
	PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains
	Noureen, Naila; Cheema, Mohsin Tassawar; Anwar, Sumaira; Hasnain, Shahida; Sajid, Imran
通讯作者	Sajid, Imran
来源期刊	POLISH JOURNAL OF MICROBIOLOGY
ISSN	1733-1331
EISSN	2544-4646
出版年	2020
卷号	69 期号:2 页码:139-149
英文摘要	This study aimed to investigate the PCR-based screening strategy for the prediction of the antimicrobial biosynthetic potential of the selected Streptomyces strains originated from an extreme environment (Cholistan Desert, Pakistan). The biosynthetic potential was determined by using both molecular and culture-dependent screening approaches. The four biosynthetic genes clusters, including the pks-1, nrps, cyp P450 hydroxylase (cyps), and glycopeptide oxy b genes, were investigated in the selected strains by PCR amplification, sequencing, and by subsequent bioinformatics approaches. Among the 40 selected Streptomyces strains, 33 strains possessed the nips gene, 17 strains carried the pks- I gene, four strains were found to have the cyps gene, and none of the strain carried oxy b gene. The Streptomyces strains including NR-1, NR-10, NR-14, and NR-15 were investigated for in vitro antifungal activity against Fusarium oxysporum, Rhizoctonia solani, and Aspergillus sp. The extracts were analyzed for chemical profiling (TLC and HPLC-UV), and a unique pattern of secondary metabolites was observed. The selected strains exhibited pronounced antifungal activity against the fungal test strains with the zone of inhibition up to 17, 18, and 19 mm, respectively. The study depicts that gene-based screening can be successfully applied to Identify potentially bioactive strains by usin a single screening process. This PCR-based approach is rapid and can be used for sorting out and selecting the potential candidate among actinobacterial culture collections. Such a preselection or strain prioritization consequently decreases the time and efforts required for selecting the potential bioactive strain, which then can be subjected to the detailed chemical analysis.
英文关键词	gene-based screening polyene specific cytochrome P450 hydroxylase (CYP) nrps pks-1 Streptomyces
类型	Article
语种	英语
国家	Pakistan
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000543484700002
WOS关键词	NONRIBOSOMAL PEPTIDE SYNTHETASE ; POLYKETIDE SYNTHASE GENE ; STREPTOMYCES-AVERMITILIS ; CLUSTER ; ANTIBACTERIAL ; METABOLITE ; SYSTEMS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319737
作者单位	Univ Punjab, Dept Microbiol & Mol Genet, Quid I Azam Campus, Lahore, Pakistan
推荐引用方式 GB/T 7714	Noureen, Naila,Cheema, Mohsin Tassawar,Anwar, Sumaira,et al. PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains[J],2020,69(2):139-149.
APA	Noureen, Naila,Cheema, Mohsin Tassawar,Anwar, Sumaira,Hasnain, Shahida,&Sajid, Imran.(2020).PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains.POLISH JOURNAL OF MICROBIOLOGY,69(2),139-149.
MLA	Noureen, Naila,et al."PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains".POLISH JOURNAL OF MICROBIOLOGY 69.2(2020):139-149.