干旱区生态环境知识资源中心(Arid): The Genome Analysis ofMethylobacterium populiYC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase

Arid

DOI	10.3390/ijms21124436
	The Genome Analysis ofMethylobacterium populiYC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase
	Li, Xianjun 1; Wang, Junhuan 1; Jia, Yang 1; Reheman, Aikebaier 2; Yan, Yanchun 1
通讯作者	Reheman, Aikebaier ; Yan, Yanchun
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2020
卷号	21 期号:12
英文摘要	Methylobacterium populiYC-XJ1 isolated from desert soil exhibited a diverse degrading ability towards aromatic oxyphenoxypropionic acid esters (AOPPs) herbicide, phthalate esters (PAEs), organophosphorus flame retardants (OPFRs), chlorpyrifos and phoxim. The genome of YC-XJ1 was sequenced and analyzed systematically. YC-XJ1 contained a large number of exogenous compounds degradation pathways and hydrolase resources. The quizalofop-p-ethyl (QPE) degrading geneqpeh2and diethyl phthalate (DEP) degrading genedeph1were cloned and expressed. The characteristics of corresponding hydrolases were investigated. The specific activity of recombinant QPEH2 was 0.1 +/- 0.02 U mg(-1)for QPE withk(cat)/K(m)values of 1.8 +/- 0.016 (mM(-1)center dot s(-1)). The specific activity of recombinant DEPH1 was 0.1 +/- 0.02 U mg(-1)for DEP withk(cat)/K(m)values of 0.8 +/- 0.02 (mM(-)(1)center dot s(-)(1)). This work systematically illuminated the metabolic versatility of strain YC-XJ1 via the combination of genomics analysis and laboratory experiments. These results suggested that strain YC-XJ1 with diverse xenobiotics biodegrading capacity was a promising candidate for the bioremediation of polluted sites.
英文关键词	Methylobacterium populi genome sequencing hydrolase quizalofop-p-ethyl diethyl phthalate degradation
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000550158900001
WOS关键词	QUIZALOFOP-P-ETHYL ; ORGANOPHOSPHATE FLAME RETARDANTS ; N-BUTYL PHTHALATE ; DIETHYL PHTHALATE ; TRIPHENYL PHOSPHATE ; GENE ; TOXICITY ; CLONING ; EXPOSURE ; WATER
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319610
作者单位	1.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China; 2.Ningde Normal Univ, Key Lab Toxicol, Ningde 352100, Peoples R China
推荐引用方式 GB/T 7714	Li, Xianjun,Wang, Junhuan,Jia, Yang,et al. The Genome Analysis ofMethylobacterium populiYC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase[J],2020,21(12).
APA	Li, Xianjun,Wang, Junhuan,Jia, Yang,Reheman, Aikebaier,&Yan, Yanchun.(2020).The Genome Analysis ofMethylobacterium populiYC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(12).
MLA	Li, Xianjun,et al."The Genome Analysis ofMethylobacterium populiYC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.12(2020).