干旱区生态环境知识资源中心(Arid): Complete plastome sequencing resolves taxonomic relationships among species of Calligonum L. (Polygonaceae) in China

Arid

DOI	10.1186/s12870-020-02466-5
	Complete plastome sequencing resolves taxonomic relationships among species of Calligonum L. (Polygonaceae) in China
	Song, Feng 1,2; Li, Ting 1,3; Burgess, Kevin S.4; Feng, Ying 2,5; Ge, Xue-Jun 3
通讯作者	Feng, Ying ; Ge, Xue-Jun
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2020
卷号	20 期号:1
英文摘要	Background Calligonum(Polygonaceae) is distributed from southern Europe through northern Africa to central Asia, and is typically found in arid, desert regions. Previous studies have revealed that standard DNA barcodes fail to discriminateCalligonumspecies. In this study, the complete plastid genomes (plastome) for 32 accessions of 21Calligonumspecies is sequenced to not only generate the first complete plastome sequence for the genusCalligonumbut to also 1) Assess the ability of the complete plastome sequence to discern species within the group, and 2) screen the plastome sequence for a cost-effective DNA barcode that can be used in future studies to resolve taxonomic relationships within the group. Results The whole plastomes ofCalligonumspecies possess a typical quadripartite structure. The size of theCalligonumplastome is approximately 161 kilobase pairs (kbp), and encodes 113 genes, including 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Based on ML phylogenetic tree analyses, the complete plastome has higher species identification (78%) than combinations of standard DNA barcodes (rbcL + matK + nrITS, 56%). Five newly screened gene regions (ndhF,trnS-G,trnC-petN,ndhF-rpl32,rpl32-trnL) had high species resolution, wherendhFandtrnS-Gwere able to distinguish the highest proportion ofCalligonumspecies (56%). Conclusions The entire plastid genome was the most effective barcode for the genusCalligonum, although other gene regions showed great potential as taxon-specific barcodes for species identification inCalligonum.
英文关键词	Calligonum DNA barcodes Plastid genome Species resolution
类型	Article
语种	英语
国家	Peoples R China ; USA
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000541756500002
WOS关键词	COMPLETE CHLOROPLAST GENOME ; EVOLUTIONARY DYNAMICS ; PHYLOGENETIC ANALYSIS ; SUPER-BARCODES ; DNA ; TOOL ; ANNOTATION ; ALIGNMENT ; FAILURE ; REVEALS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319444
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 83011, Peoples R China; 3.Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Peoples R China; 4.Columbus State Univ, Univ Syst Georgia, Dept Biol, Columbus, GA 31907 USA; 5.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Specimen Museum, Urumqi 83011, Peoples R China
推荐引用方式 GB/T 7714	Song, Feng,Li, Ting,Burgess, Kevin S.,et al. Complete plastome sequencing resolves taxonomic relationships among species of Calligonum L. (Polygonaceae) in China[J]. 中国科学院新疆生态与地理研究所,2020,20(1).
APA	Song, Feng,Li, Ting,Burgess, Kevin S.,Feng, Ying,&Ge, Xue-Jun.(2020).Complete plastome sequencing resolves taxonomic relationships among species of Calligonum L. (Polygonaceae) in China.BMC PLANT BIOLOGY,20(1).
MLA	Song, Feng,et al."Complete plastome sequencing resolves taxonomic relationships among species of Calligonum L. (Polygonaceae) in China".BMC PLANT BIOLOGY 20.1(2020).