Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s11696-020-01160-y |
Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect | |
Trivedi, Vikas1; Shah, Priyanka A.2; Shrivastav, Pranav S.2; Sanyal, Mallika3 | |
通讯作者 | Shrivastav, Pranav S. |
来源期刊 | CHEMICAL PAPERS
![]() |
ISSN | 2585-7290 |
EISSN | 1336-9075 |
出版年 | 2020 |
卷号 | 74期号:12页码:4365-4378 |
英文摘要 | Interference of endogenous components present in biological fluids has a profound effect on the performance of LC-MS/MS-based assays. The present work evaluates the effect of plasma matrix components during sample preparation for accurate and precise determination of valsartan, clindamycin and mesalamine by LC-ESI-MS/MS. The methods were thoroughly assessed using different approaches for sample preparation such as solid-phase extraction (SPE) with different cartridges for valsartan, conventional protein precipitation and Phree phospholipid removal cartridge for clindamycin and liquid-liquid extraction (LLE) with and without derivatization for mesalamine to minimize matrix interference. The extent of matrix effect was determined by post-column analyte infusion, post-extraction spike method and by calculation of slope of calibration lines for different plasma sources. The influence of matrix ion suppression/enhancement on the extraction recovery and sensitivity of the methods was identified and quantified. The mean recovery obtained was 96.8% for valsartan using SPE on Oasis MCX, 92.2% for clindamycin using Phree phospholipid cartridge and 96.5% for mesalamine using LLE following derivatization. The intra-batch and inter-day precision (% CV) was <4.0 for the methods and was validated as per the current regulatory guidance. |
英文关键词 | Valsartan Clindamycin Mesalamine Sample preparation Matrix effect LC-ESI-MS MS |
类型 | Article |
语种 | 英语 |
国家 | India |
收录类别 | SCI-E |
WOS记录号 | WOS:000540969200001 |
WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; 5-AMINOSALICYLIC ACID ; ESI-MS/MS ; HPLC METHOD ; VALIDATION ; AMLODIPINE ; METABOLITE ; HYDROCHLOROTHIAZIDE ; ASSAY |
WOS类目 | Chemistry, Multidisciplinary |
WOS研究方向 | Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/319342 |
作者单位 | 1.Kadi Sarva Viswavidyalaya, Sect 15, Ghandhinagar 382715, Gujarat, India; 2.Gujarat Univ, Sch Sci, Dept Chem, Ahmadabad 380009, Gujarat, India; 3.St Xaviers Coll, Chem Dept, Ahmadabad 380009, Gujarat, India |
推荐引用方式 GB/T 7714 | Trivedi, Vikas,Shah, Priyanka A.,Shrivastav, Pranav S.,et al. Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect[J],2020,74(12):4365-4378. |
APA | Trivedi, Vikas,Shah, Priyanka A.,Shrivastav, Pranav S.,&Sanyal, Mallika.(2020).Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect.CHEMICAL PAPERS,74(12),4365-4378. |
MLA | Trivedi, Vikas,et al."Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect".CHEMICAL PAPERS 74.12(2020):4365-4378. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。