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DOI	10.1007/s11696-020-01160-y
	Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect
	Trivedi, Vikas 1; Shah, Priyanka A.2; Shrivastav, Pranav S.2; Sanyal, Mallika 3
通讯作者	Shrivastav, Pranav S.
来源期刊	CHEMICAL PAPERS
ISSN	2585-7290
EISSN	1336-9075
出版年	2020
卷号	74 期号:12 页码:4365-4378
英文摘要	Interference of endogenous components present in biological fluids has a profound effect on the performance of LC-MS/MS-based assays. The present work evaluates the effect of plasma matrix components during sample preparation for accurate and precise determination of valsartan, clindamycin and mesalamine by LC-ESI-MS/MS. The methods were thoroughly assessed using different approaches for sample preparation such as solid-phase extraction (SPE) with different cartridges for valsartan, conventional protein precipitation and Phree phospholipid removal cartridge for clindamycin and liquid-liquid extraction (LLE) with and without derivatization for mesalamine to minimize matrix interference. The extent of matrix effect was determined by post-column analyte infusion, post-extraction spike method and by calculation of slope of calibration lines for different plasma sources. The influence of matrix ion suppression/enhancement on the extraction recovery and sensitivity of the methods was identified and quantified. The mean recovery obtained was 96.8% for valsartan using SPE on Oasis MCX, 92.2% for clindamycin using Phree phospholipid cartridge and 96.5% for mesalamine using LLE following derivatization. The intra-batch and inter-day precision (% CV) was <4.0 for the methods and was validated as per the current regulatory guidance.
英文关键词	Valsartan Clindamycin Mesalamine Sample preparation Matrix effect LC-ESI-MS MS
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000540969200001
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; 5-AMINOSALICYLIC ACID ; ESI-MS/MS ; HPLC METHOD ; VALIDATION ; AMLODIPINE ; METABOLITE ; HYDROCHLOROTHIAZIDE ; ASSAY
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319342
作者单位	1.Kadi Sarva Viswavidyalaya, Sect 15, Ghandhinagar 382715, Gujarat, India; 2.Gujarat Univ, Sch Sci, Dept Chem, Ahmadabad 380009, Gujarat, India; 3.St Xaviers Coll, Chem Dept, Ahmadabad 380009, Gujarat, India
推荐引用方式 GB/T 7714	Trivedi, Vikas,Shah, Priyanka A.,Shrivastav, Pranav S.,et al. Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect[J],2020,74(12):4365-4378.
APA	Trivedi, Vikas,Shah, Priyanka A.,Shrivastav, Pranav S.,&Sanyal, Mallika.(2020).Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect.CHEMICAL PAPERS,74(12),4365-4378.
MLA	Trivedi, Vikas,et al."Analysis of valsartan, clindamycin and mesalamine in human plasma by LC-MS/MS using different extraction methodologies to overcome matrix effect".CHEMICAL PAPERS 74.12(2020):4365-4378.