干旱区生态环境知识资源中心(Arid): Evaluating Different Methodologies for Bioprospecting Actinomycetes in Canary Islands Soils

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DOI	10.1007/s00284-020-02030-2
	Evaluating Different Methodologies for Bioprospecting Actinomycetes in Canary Islands Soils
	Hernandez-Bolanos, Eduardo 1,2; Montesdeoca-Flores, David 1; Abreu-Yanes, Estefania 1; Barrios, Milagros Leon 2; Abreu-Acosta, Nestor 1
通讯作者	Hernandez-Bolanos, Eduardo
来源期刊	CURRENT MICROBIOLOGY
ISSN	0343-8651
EISSN	1432-0991
出版年	2020
卷号	77 期号:9 页码:2510-2522
英文摘要	Actinomycetes are a wide group of Gram positive prokaryotes, the soil being their most characteristic habitat, where they play important ecological functions. Their immense biotechnological potential as producers of bioactive molecules of great commercial and industrial interest is exemplified by most antibiotics for clinical use being derived from this group of bacteria. In this work several methodologies and culture media were tested for the isolation of actinomycetes in soils from three different edafoclimatic areas of Tenerife (Canary Islands, Spain): an arid zone of the southeast coast of the island, a humid area in the laurel forest and the Canary high mountain. The results of this work evidenced that just an air drying of the sample during 7 days (pretreatment) produces high counts of actinomycetes versus non-actinomycetes in the three soils and in any of the culture media studied, making unnecessary post physical and chemical treatments. However, the only method that produced an exclusive isolation of actinomycetes was the use of 0.22-mu m filters as a physical barrier, and hence being a tool of unquestionable utility to estimate actinomycetes populations in soil. The analysis by BOX rep-PCR determined that the richness and genetic diversity of the isolates of these localities are very high. The greatest similarities were, with one exception, found between strains from the same locations. Izana was one that obtained the highest diversity. Regarding the biotecnology potential as antibiotic produces isolates from Guimar soils are the most promising as 7 out of 10 isolates showed some antibiotic activity.
类型	Article
语种	英语
国家	Spain
收录类别	SCI-E
WOS记录号	WOS:000538970100001
WOS关键词	STREPTOMYCES ; DIVERSITY
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319307
作者单位	1.NERTALAB SL, C Jose Rodriguez Moure 4, Tenerife 38008, Spain; 2.Univ La Laguna, Dept Bioquim Microbiol Biol Celular & Genet, Avda Astrofis Sanchez S-N, Tenerife 38204, Spain
推荐引用方式 GB/T 7714	Hernandez-Bolanos, Eduardo,Montesdeoca-Flores, David,Abreu-Yanes, Estefania,et al. Evaluating Different Methodologies for Bioprospecting Actinomycetes in Canary Islands Soils[J],2020,77(9):2510-2522.
APA	Hernandez-Bolanos, Eduardo,Montesdeoca-Flores, David,Abreu-Yanes, Estefania,Barrios, Milagros Leon,&Abreu-Acosta, Nestor.(2020).Evaluating Different Methodologies for Bioprospecting Actinomycetes in Canary Islands Soils.CURRENT MICROBIOLOGY,77(9),2510-2522.
MLA	Hernandez-Bolanos, Eduardo,et al."Evaluating Different Methodologies for Bioprospecting Actinomycetes in Canary Islands Soils".CURRENT MICROBIOLOGY 77.9(2020):2510-2522.