干旱区生态环境知识资源中心(Arid): Determination of four nitrofuran metabolites in gelatin Chinese medicine using dispersive solid phase extraction and pass-through solid phase extraction coupled to ultra high performance liquid chromatography-tandem mass spectrometry

Arid

DOI	10.1016/j.jchromb.2020.122018
	Determination of four nitrofuran metabolites in gelatin Chinese medicine using dispersive solid phase extraction and pass-through solid phase extraction coupled to ultra high performance liquid chromatography-tandem mass spectrometry
	Gong, Jinyan 1; Li, Jiong 2; Yuan, Haina 1; Chu, Bingquan 1; Lin, Weijie 2; Cao, Qingwen 2; Zhao, Qiqi 2; Fang, Ruosi 1; Li, Ling 1; Xiao, Gongnian 1
通讯作者	Li, Jiong
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2020
卷号	1146
英文摘要	This study established a validated analytical method for the first time on the determination of nitrofuran metabolites, including semicarbazide (SEM), 1-aminohydantoin (AHD), 3-amino-2-oxazolidinone (AOZ) and 3-amino-5-morpholinomethyl-2-oxazolinone (AMOZ) in gelatin Chinese medicine. A C-18 column with the mobile phase consisting of acetonitrile and 5 mmol/L ammonium acetate in water was used to separate these nitrofuran metabolites. The limit of detection of SEM, AHD, AOZ and AMOZ were found to be 0.2 mu g/kg, 0.3 mu g/kg, 0.2 mu g/kg and 0.2 mu g/kg, whereas their limit of quantification were 0.6 mu g/kg, 0.8 mu g/kg, 0.6 mu g/kg and 0.5 mu g/kg. These nitrofuran metabolites exhibited a good linear standard curve (regression coefficients above 0.99) with a concentration range of 2 mu g/L to 100 mu g/L. Regarding extraction procedure, gelatin Chinese medicine was pre-treated with pepsin and then extracted using 5% formic acid (v/v) in acetonitrile. The resultant extract was purified through dispersive solid phase extraction using 1000 mg anhydrous sodium sulfate, 300 mg octadecyl carbon silica gel sorbent absorbent and 500 mg ethylenediamine-N-propyl carbon silica gel absorbent, and then further purified on Oasis PRiME HLB cartridges. The matrix effect was effectively eliminated after the clean-up procedure as confirmed by comparing the ratio of standard curves prepared by standards dissolved in both matrix solvent and 5 mmol/L ammonium acetate in water: acetonitrile (95:5, v/v). The recoveries of these nitrofuran metabolites under the 1 mu g/kg, 2 mu g/kg and 10 mu g/kg spiking levels were between 77.4% and 95.6%. These metabolites after the extraction were stable at 4 degrees C for 24 h. The validated method was used to analyze the residue level of these nitrofuran metabolites in 25 gelatin Chinese medicines. Results showed that only one Colla Corii Asini sample contained SEM (2.52 mu g/kg) and AOZ (6.27 mu g/kg), whereas one Testudinis Carapacis et Plastri sample had SEM (1.27 mu g/kg) and AMOZ (9.53 mu g/kg).
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000534279400001
WOS关键词	ANIMAL FEEDS ; SHRIMP ; FURAZOLIDONE ; RESIDUES ; UV ; PESTICIDES ; MUSCLE ; FOOD
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319289
作者单位	1.Zhejiang Univ Sci & Technol, Sch Biol & Chem Engn, Zhejiang Prov Key Lab Biol & Chem Proc Technol Fa, Hangzhou 310023, Zhejiang, Peoples R China; 2.Hangzhou Inst Food & Drug Control, Hangzhou 310017, Peoples R China
推荐引用方式 GB/T 7714	Gong, Jinyan,Li, Jiong,Yuan, Haina,et al. Determination of four nitrofuran metabolites in gelatin Chinese medicine using dispersive solid phase extraction and pass-through solid phase extraction coupled to ultra high performance liquid chromatography-tandem mass spectrometry[J],2020,1146.
APA	Gong, Jinyan.,Li, Jiong.,Yuan, Haina.,Chu, Bingquan.,Lin, Weijie.,...&Xiao, Gongnian.(2020).Determination of four nitrofuran metabolites in gelatin Chinese medicine using dispersive solid phase extraction and pass-through solid phase extraction coupled to ultra high performance liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1146.
MLA	Gong, Jinyan,et al."Determination of four nitrofuran metabolites in gelatin Chinese medicine using dispersive solid phase extraction and pass-through solid phase extraction coupled to ultra high performance liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1146(2020).