干旱区生态环境知识资源中心(Arid): Deciphering the Root Endosphere Microbiome of the Desert Plant Alhagi sparsifolia for Drought Resistance-Promoting Bacteria

Arid

DOI	10.1128/AEM.02863-19
	Deciphering the Root Endosphere Microbiome of the Desert Plant Alhagi sparsifolia for Drought Resistance-Promoting Bacteria
	Zhang, Lei 1; Zhang, Weipeng 2; Li, Qiqi 1; Cui, Rui 1; Wang, Zhuo 1; Wang, Yao 1; Zhang, Yu-Zhong 2; Ding, Wei 2; Shen, Xihui 1
通讯作者	Ding, Wei ; Shen, Xihui
来源期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
EISSN	1098-5336
出版年	2020
卷号	86 期号:11
英文摘要	Drought is among the most destructive abiotic stresses limiting crop growth and yield worldwide. Although most research has focused on the contribution of plant-associated microbial communities to plant growth and disease suppression, far less is known about the microbes involved in drought resistance among desert plants. In the present study, we applied 16S rRNA gene amplicon sequencing to determine the structure of rhizosphere and root endosphere microbiomes of Alhagi sparsifolia. Compared to those of the rhizosphere, endosphere microbiomes had lower diversity but contained several taxa with higher relative abundance; many of these taxa were also present in the roots of other desert plants. We isolated a Pseudomonas strain (LTGT-11-2Z) that was prevalent in root endosphere microbiomes of A. sparsifolia and promoted drought resistance during incubation with wheat. Complete genome sequencing of LTGT-11- 2Z revealed 1-aminocyclopropane-1-carboxylate deaminases, siderophore, spermidine, and colanic acid biosynthetic genes, as well as type VI secretion system (T6SS) genes, which are likely involved in biofilm formation and plant-microbe interactions. Together, these results indicate that drought-enduring plants harbor bacterial endophytes favorable to plant drought resistance, and they suggest that novel endophytic bacterial taxa and gene resources may be discovered among these desert plants. IMPORTANCE Understanding microbe-mediated plant resistance to drought is important for sustainable agriculture. We performed 16S rRNA gene amplicon sequencing and culture-dependent functional analyses of Alhagi sparsifolia rhizosphere and root endosphere microbiomes and identified key endophytic bacterial taxa and their genes facilitating drought resistance in wheat. This study improves our understanding of plant drought resistance and provides new avenues for drought resistance improvement in crop plants under field conditions.
英文关键词	Alhagi sparsifolia drought resistance Pseudomonas root endosphere
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000539019800027
WOS关键词	COMPLETE GENOME SEQUENCE ; ENDOPHYTIC COLONIZATION ; ACC DEAMINASE ; GROWTH ; STRESS ; COMMUNITIES ; TOLERANCE ; DATABASE ; GENE ; L.
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319234
作者单位	1.Northwest A&F Univ, Shaanxi Key Lab Agr & Environm Microbiol, State Key Lab Crop Stress Biol Arid Areas, Coll Life Sci, Yangling, Shaanxi, Peoples R China; 2.Ocean Univ China, Coll Marine Life Sci, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lei,Zhang, Weipeng,Li, Qiqi,et al. Deciphering the Root Endosphere Microbiome of the Desert Plant Alhagi sparsifolia for Drought Resistance-Promoting Bacteria[J]. 西北农林科技大学,2020,86(11).
APA	Zhang, Lei.,Zhang, Weipeng.,Li, Qiqi.,Cui, Rui.,Wang, Zhuo.,...&Shen, Xihui.(2020).Deciphering the Root Endosphere Microbiome of the Desert Plant Alhagi sparsifolia for Drought Resistance-Promoting Bacteria.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,86(11).
MLA	Zhang, Lei,et al."Deciphering the Root Endosphere Microbiome of the Desert Plant Alhagi sparsifolia for Drought Resistance-Promoting Bacteria".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 86.11(2020).