干旱区生态环境知识资源中心(Arid): Achromobacter sp. FB-14 harboring ACC deaminase activity augmented rice growth by upregulating the expression of stress-responsive CIPK genes under salinity stress

Arid

DOI	10.1007/s42770-019-00199-8
	Achromobacter sp. FB-14 harboring ACC deaminase activity augmented rice growth by upregulating the expression of stress-responsive CIPK genes under salinity stress
	Shahid, Muhammad 1; Shah, Asad Ali 1; Basit, Farwa 1,2; Noman, Muhammad 1,3; Zubair, Muhammad 1; Ahmed, Temoor 1,3; Naqqash, Tahir 4; Manzoor, Irfan 1; Maqsood, Awais 1
通讯作者	Shah, Asad Ali
来源期刊	BRAZILIAN JOURNAL OF MICROBIOLOGY
ISSN	1517-8382
EISSN	1678-4405
出版年	2020
卷号	51 期号:2 页码:719-728
英文摘要	Soil salinity is one of the major plant growth and yield-limiting constraints in arid and semi-arid regions of the world. In addition to the oxidative damage, increasing salt stress is associated with elevated cellular ethylene levels due to the synthesis of 1-aminocyclopropane-1-carboxylic acid (ACC) in large amounts. The objective of the current study was to elucidate the inoculation effect of an ACC deaminase (ACCD)-producing phytobeneficial strain Achromobacter sp. FB-14 on rice plants to alleviate the salinity effects by upregulation of the stress-responsive CIPK genes. The strain FB-14 was isolated by using nutrient agar medium at 855 mM NaCl concentration and it was taxonomically identified as Achromobacter sp. with more than 99% 16S rRNA gene sequence similarity with many Achromobacter species. The strain FB-14 demonstrated substantial in vitro potential for ACCD activity, synthesis of indole compounds, and phosphate solubilization up to 100 mM NaCl concentration in the culture medium. The gene corresponding to ACCD activity (acdS) was amplified and sequenced in order to confirm the inherent enzyme activity of the strain at a molecular level. The rifampicin-resistant derivative of strain FB-14 was recovered from the rice rhizosphere on antibiotic medium up to 21 days of sowing. Moreover, the strain FB-14 was inoculated on rice plants under salinity and it not only enhanced the growth of rice plants in terms of root and shoot length, and fresh and dry weight, but also upregulated the expression of stress-responsive CIPK genes (OsCIPK03, OsCIPK12, and OsCIPK15) according to the results of qRT-PCR analysis. To the best of our knowledge, this is the first report deciphering the role of plant-beneficial Achromobacter strain relieving the rice plants from salt stress by promoting the growth and enhancing the expression of stress-responsive CIPK genes.
英文关键词	ACC deaminase Gene expression PGPR Rice Salt tolerance
类型	Article
语种	英语
国家	Pakistan ; Peoples R China
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000530831200028
WOS关键词	PLANT-GROWTH ; SALT TOLERANCE ; BACTERIA ; ENHANCEMENT ; RESISTANCE ; YIELD ; SOIL ; RHIZOBACTERIA ; RHIZOSPHERE ; PSEUDOMONAS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319228
作者单位	1.Govt Coll Univ, Dept Bioinformat & Biotechnol, Faisalabad 38000, Pakistan; 2.Zhejiang Univ, Coll Agr & Biotechnol, Inst Crop Sci, Hangzhou 310058, Peoples R China; 3.Zhejiang Univ, Inst Biotechnol, Natl Key Lab Rice Biol, Hangzhou 310058, Peoples R China; 4.Bahauddin Zakariya Univ, Inst Mol Biol & Biotechnol, Multan, Pakistan
推荐引用方式 GB/T 7714	Shahid, Muhammad,Shah, Asad Ali,Basit, Farwa,et al. Achromobacter sp. FB-14 harboring ACC deaminase activity augmented rice growth by upregulating the expression of stress-responsive CIPK genes under salinity stress[J],2020,51(2):719-728.
APA	Shahid, Muhammad.,Shah, Asad Ali.,Basit, Farwa.,Noman, Muhammad.,Zubair, Muhammad.,...&Maqsood, Awais.(2020).Achromobacter sp. FB-14 harboring ACC deaminase activity augmented rice growth by upregulating the expression of stress-responsive CIPK genes under salinity stress.BRAZILIAN JOURNAL OF MICROBIOLOGY,51(2),719-728.
MLA	Shahid, Muhammad,et al."Achromobacter sp. FB-14 harboring ACC deaminase activity augmented rice growth by upregulating the expression of stress-responsive CIPK genes under salinity stress".BRAZILIAN JOURNAL OF MICROBIOLOGY 51.2(2020):719-728.