干旱区生态环境知识资源中心(Arid): Identification of drought resistant miRNA in Macleaya cordata by high-throughput sequencing

Arid

DOI	10.1016/j.abb.2020.108300
	Identification of drought resistant miRNA in Macleaya cordata by high-throughput sequencing
	Yu, Linlan 2; Zhou, Li 2; Liu, Wei 1,3; Huang, Peng 1,2; Jiang, Ruolan 1,2; Tang, Zhaoshan 5; Cheng, Pi 1,2; Zeng, Jianguo 1,4
通讯作者	Cheng, Pi ; Zeng, Jianguo
来源期刊	ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
ISSN	0003-9861
EISSN	1096-0384
出版年	2020
卷号	684
英文摘要	Drought is one of the most serious factors affecting crop yields in the world. Macleaya cordata (Willd.) is a draught-tolerant medicinal plant that has been proposed as a pioneer crop to be cultivated in arid areas. However, the exact molecular mechanisms through which M. cordata responds to draught stress remain elusive. In recent years, microRNA (miRNAs) in plants have been associated with stress response. Based on these findings, the current study aimed to shed light on the potential regulatory roles of miRNAs in the draught tolerance of M. cordata by employing high-throughput RNA sequencing and degradation sequencing. Six M. cordata plants were randomly divided into two equal experiment groups, including one draught group and one control group. High-throughput sequencing of the M. cordata samples led to the identification of 895 miRNAs, of which 18 showed significantly different expression levels between the two groups. PsRobot analysis and degradation sequencing predicted the differential miRNAs to target 59 and 36 genes, respectively. Functional analysis showed that 38 of the predicted genes could be implicated in the modulation of stress response. Four miRNAs and eight target genes were selected for quantitative real-time polymerase chain reaction (qRT-PCR) validation. The expression trend of each miRNA analyzed by qRT-PCR was consistent with that determined by sequencing, and was negatively correlated with those of its target genes. The results of our current study supported the involvement of miRNAs in the draught tolerance of M. cordata and could pave the way for further investigation into the related regulatory mechanisms.
英文关键词	Macleaya cordata miRNAs drought stress High-throughput sequencing Degradation sequencing
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000525451100002
WOS关键词	STRESS-REGULATED MICRORNAS ; GENE-EXPRESSION ; SMALL RNAS ; TOLERANCE ; GLUTATHIONE ; SATIVA ; PLANTS
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319115
作者单位	1.Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China; 2.Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China; 3.Hunan Agr Univ, Ctr Analyt Serv, Changsha 410208, Peoples R China; 4.Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China; 5.Micolta Bioresource Inc, Changsha 410016, Peoples R China
推荐引用方式 GB/T 7714	Yu, Linlan,Zhou, Li,Liu, Wei,et al. Identification of drought resistant miRNA in Macleaya cordata by high-throughput sequencing[J],2020,684.
APA	Yu, Linlan.,Zhou, Li.,Liu, Wei.,Huang, Peng.,Jiang, Ruolan.,...&Zeng, Jianguo.(2020).Identification of drought resistant miRNA in Macleaya cordata by high-throughput sequencing.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,684.
MLA	Yu, Linlan,et al."Identification of drought resistant miRNA in Macleaya cordata by high-throughput sequencing".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 684(2020).