干旱区生态环境知识资源中心(Arid): A new collar rot disease of cowpea (Vigna unguiculata) caused by Aplosporella hesperidica in India

Arid

DOI	10.1111/lam.13293
	A new collar rot disease of cowpea (Vigna unguiculata) caused by Aplosporella hesperidica in India
	Deepika, Y. S.1,2; Mahadevakumar, S.1; Amruthesh, K. N.1; Lakshmidevi, N.2
通讯作者	Lakshmidevi, N.
来源期刊	LETTERS IN APPLIED MICROBIOLOGY
ISSN	0266-8254
EISSN	1472-765X
出版年	2020
卷号	71 期号:2 页码:154-163
英文摘要	Cowpea is an important pulse crop cultivated in arid and semi-arid regions of the world. During field survey, a characteristic wilt was observed in around 45 ha of cowpea fields with incidence 17-25%. Infection was seen in pre-flowering stage and infected plants showed quick wilt symptoms with tan lesions near the stem-soil interface. Fungal pathogens associated were isolated on PDA, which produced dark to grey olivaceous colonies in the centre, and aerial mycelia were appressed with floccose and white to smoke-grey. Conidia are aseptate, initially hyaline, smooth-walled, broadly ellipsoidal with rounded ends becoming dark brown. Based on these morphological features, the fungal pathogen was identified as Aplosporella sp. The ITS-rDNA region was amplified using ITS1/ITS4 primers and sequenced. The nBLAST and phylogenetic analysis confirmed the pathogen as Aplosporella hesperidica. The Koch's postulates were performed on 45-days-old cowpea plants with mycelial disc of A. hesperidica. Development of typical necrotic lesions was observed after 28 days of post-inoculation and the pathogen's identity was confirmed based on re-isolation. Efficacy of fungicides evaluated in vitro showed that the pathogen is highly sensitive to systemic fungicides rather than the contact fungicides. The cowpea production was severely affected owing to the causative agent A. hesperidica. The collar rot disease of cowpea by A. hesperidica is the first report in India.
英文关键词	Aplosporella hesperidica ITS-rDNA new disease report pathogenicity pulses
类型	Article
语种	英语
国家	India
开放获取类型	Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000530373400001
WOS关键词	1ST REPORT ; COLLETOTRICHUM ; HOSTS ; STEM ; FAMILIES ; GENERA ; FUNGI ; YIELD ; ROOT ; L.
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319064
作者单位	1.Univ Mysore, Dept Studies Bot, Mysuru, Karnataka, India; 2.Univ Mysore, Dept Studies Microbiol, Mysuru 570006, Karnataka, India
推荐引用方式 GB/T 7714	Deepika, Y. S.,Mahadevakumar, S.,Amruthesh, K. N.,et al. A new collar rot disease of cowpea (Vigna unguiculata) caused by Aplosporella hesperidica in India[J],2020,71(2):154-163.
APA	Deepika, Y. S.,Mahadevakumar, S.,Amruthesh, K. N.,&Lakshmidevi, N..(2020).A new collar rot disease of cowpea (Vigna unguiculata) caused by Aplosporella hesperidica in India.LETTERS IN APPLIED MICROBIOLOGY,71(2),154-163.
MLA	Deepika, Y. S.,et al."A new collar rot disease of cowpea (Vigna unguiculata) caused by Aplosporella hesperidica in India".LETTERS IN APPLIED MICROBIOLOGY 71.2(2020):154-163.