Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1111/1755-0998.13148 |
| Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding | |
| van der Heyde, Mieke1,2; Bunce, Michael2; Wardell-Johnson, Grant1; Fernandes, Kristen2; White, Nicole E.2; Nevill, Paul1,2 | |
| 通讯作者 | van der Heyde, Mieke |
| 来源期刊 | MOLECULAR ECOLOGY RESOURCES
 |
| ISSN | 1755-098X |
| EISSN | 1755-0998 |
| 出版年 | 2020 |
| 卷号 | 20期号:3 |
| 英文摘要 | Biological surveys based on visual identification of the biota are challenging, expensive and time consuming, yet crucial for effective biomonitoring. DNA metabarcoding is a rapidly developing technology that can also facilitate biological surveys. This method involves the use of next generation sequencing technology to determine the community composition of a sample. However, it is uncertain as to what biological substrate should be the primary focus of metabarcoding surveys. This study aims to test multiple sample substrates (soil, scat, plant material and bulk arthropods) to determine what organisms can be detected from each and where they overlap. Samples (n = 200) were collected in the Pilbara (hot desert climate) and Swan Coastal Plain (hot Mediterranean climate) regions of Western Australia. Soil samples yielded little plant or animal DNA, especially in the Pilbara, probably due to conditions not conducive to long-term preservation. In contrast, scat samples contained the highest overall diversity with 131 plant, vertebrate and invertebrate families detected. Invertebrate and plant sequences were detected in the plant (86 families), pitfall (127 families) and vane trap (126 families) samples. In total, 278 families were recovered from the survey, 217 in the Swan Coastal Plain and 156 in the Pilbara. Aside from soil, 22%-43% of the families detected were unique to the particular substrate, and community composition varied significantly between substrates. These results demonstrate the importance of selecting appropriate metabarcoding substrates when undertaking terrestrial surveys. If the aim is to broadly capture all biota then multiple substrates will be required. |
| 英文关键词 | biodiversity biological audit DNA barcoding metagenomics terrestrial |
| 类型 | Article |
| 语种 | 英语 |
| 国家 | Australia |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000529782200011 |
| WOS关键词 | SOIL ; COMMUNITIES ; IDENTIFICATION ; ARTHROPODS ; RESOURCES ; DIVERSITY ; EFFICIENT ; PLANT ; DIET |
| WOS类目 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/319019 |
| 作者单位 | 1.Curtin Univ, ARC Ctr Mine Site Restorat, Sch Mol & Life Sci, Perth, WA, Australia; 2.Curtin Univ, Trace & Environm DNA Lab, Sch Life & Mol Sci, Perth, WA, Australia |
| 推荐引用方式 GB/T 7714 | van der Heyde, Mieke,Bunce, Michael,Wardell-Johnson, Grant,et al. Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding[J],2020,20(3). |
| APA | van der Heyde, Mieke,Bunce, Michael,Wardell-Johnson, Grant,Fernandes, Kristen,White, Nicole E.,&Nevill, Paul.(2020).Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding.MOLECULAR ECOLOGY RESOURCES,20(3). |
| MLA | van der Heyde, Mieke,et al."Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding".MOLECULAR ECOLOGY RESOURCES 20.3(2020). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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