干旱区生态环境知识资源中心(Arid): Phages Actively Challenge Niche Communities in Antarctic Soils

Arid

DOI	10.1128/mSystems.00234-20
	Phages Actively Challenge Niche Communities in Antarctic Soils
	Bezuidt, Oliver K., I 1; Lebre, Pedro Humberto 1; Pierneef, Rian 1,2; Leon-Sobrino, Carlos 1; Adriaenssens, Evelien M.3; Cowan, Don A.1; Van de Peer, Yves 1,4,5; Makhalanyane, Thulani P.1
通讯作者	Makhalanyane, Thulani P.
来源期刊	MSYSTEMS
ISSN	2379-5077
出版年	2020
卷号	5 期号:3
英文摘要	By modulating the structure, diversity, and trophic outputs of microbial communities, phages play crucial roles in many biomes. In oligotrophic polar deserts, the effects of katabatic winds, constrained nutrients, and low water availability are known to limit microbial activity. Although phages may substantially govern trophic interactions in cold deserts, relatively little is known regarding the precise ecological mechanisms. Here, we provide the first evidence of widespread antiphage innate immunity in Antarctic environments using metagenomic sequence data from hypolith communities as model systems. In particular, immunity systems such as DISARM and BREX are shown to be dominant systems in these communities. Additionally, we show a direct correlation between the CRISPR-Cas adaptive immunity and the metavirome of hypolith communities, suggesting the existence of dynamic host-phage interactions. In addition to providing the first exploration of immune systems in cold deserts, our results suggest that phages actively challenge niche communities in Antarctic polar deserts. We provide evidence suggesting that the regulatory role played by phages in this system is an important determinant of bacterial host interactions in this environment. IMPORTANCE In Antarctic environments, the combination of both abiotic and biotic stressors results in simple trophic levels dominated by microbiomes. Although the past two decades have revealed substantial insights regarding the diversity and structure of microbiomes, we lack mechanistic insights regarding community interactions and how phages may affect these. By providing the first evidence of widespread antiphage innate immunity, we shed light on phage-host dynamics in Antarctic niche communities. Our analyses reveal several antiphage defense systems, including DISARM and BREX, which appear to dominate in cold desert niche communities. In contrast, our analyses revealed that genes which encode antiphage adaptive immunity were underrepresented in these communities, suggesting lower infection frequencies in cold edaphic environments. We propose that by actively challenging niche communities, phages play crucial roles in the diversification of Antarctic communities.
英文关键词	Antarctic soils archaea bacteria hypoliths phages viromics
类型	Article
语种	英语
国家	South Africa ; England ; Belgium
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000531078500001
WOS关键词	MICROBIAL COMMUNITIES ; FUNCTIONAL-CAPACITY ; CLASSIFICATION ; DIVERSITY ; ECOLOGY ; RESISTANCE ; VIRUSES ; HOT ; MICROORGANISMS ; COEVOLUTION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319018
作者单位	1.Univ Pretoria, Ctr Microbial Ecol & Genom, Dept Biochem Genet & Microbiol, Pretoria, South Africa; 2.Agr Res Council, Biotechnol Platform, Pretoria, South Africa; 3.Quadram Inst Biosci, Norwich Res Pk, Norwich, Norfolk, England; 4.Univ Ghent, Dept Plant Biotechnol & Bioinformat, Ghent, Belgium; 5.VIB, Ctr Plant Syst Biol, Ghent, Belgium
推荐引用方式 GB/T 7714	Bezuidt, Oliver K., I,Lebre, Pedro Humberto,Pierneef, Rian,et al. Phages Actively Challenge Niche Communities in Antarctic Soils[J],2020,5(3).
APA	Bezuidt, Oliver K., I.,Lebre, Pedro Humberto.,Pierneef, Rian.,Leon-Sobrino, Carlos.,Adriaenssens, Evelien M..,...&Makhalanyane, Thulani P..(2020).Phages Actively Challenge Niche Communities in Antarctic Soils.MSYSTEMS,5(3).
MLA	Bezuidt, Oliver K., I,et al."Phages Actively Challenge Niche Communities in Antarctic Soils".MSYSTEMS 5.3(2020).