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DOI	10.1007/s00203-020-01904-0
	Biochemical, functional and molecular characterization of pigeon pea rhizobia isolated from semi-arid regions of India
	Jain, Devendra 1; Kumari, Anisha 1; Saheewala, Heena 1; Sanadhya, Suman 1; Maheshwari, Deepali 1; Meena, Ram Hari 2; Singh, Abhijeet 3; Gera, Rajesh 4; Mohanty, S. R.5
通讯作者	Jain, Devendra
来源期刊	ARCHIVES OF MICROBIOLOGY
ISSN	0302-8933
EISSN	1432-072X
出版年	2020
卷号	202 期号:7 页码:1809-1816
英文摘要	Pigeon pea (Cajanus cajan (L.) Millspaugh) is among the top ten legumes grown globally not only having high tolerance to environmental stresses along, but also has the high biomass and productivity with optimal nutritional profiles. In the present study, 55 isolates of rhizobia were identified from 22 nodule samples of pigeon pea collected from semi-arid regions of India on the basis of morphological, biochemical, plant growth promoting activities and their ability to tolerate the stress conditions viz. pH, salt, temperature and drought stress. Amongst all the 55 isolates, 37 isolates showed effective nodulation under in vitro conditions in pigeon pea. Further, five isolates having multiple PGP activities and high in vitro symbiotic efficiency were subjected to 16S rRNA sequencing and confirmed their identities as Rhizobium, Mesorhizobium, Sinorhizobium sp. Further these 37 isolates were characterized at molecular level using ARDRA and revealed significant molecular diversity. Based on UPGMA clustering analysis, these isolates showed significant molecular diversity. The high degree of molecular diversity is due to mixed cropping of legumes in the region. The assessment of genetic diversity and molecular characterization of novel strains is a very important tool for the replacement of ineffective rhizobial strains with the efficient strains for the improvement in the nodulation and pigeon pea quality. The pigeon pea isolates with multiple PGPR activities could be further used for commercial production.
英文关键词	Indigenous pigeon pea rhizobia Biochemical and molecular characterization Plant growth promoting (PGP) activities In vitro symbiotic efficacy
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000534468200002
WOS关键词	CAJANUS-CAJAN ; GENETIC DIVERSITY ; GROWTH ; BRADYRHIZOBIUM ; NODULATION ; ALIGNMENT ; NITROGEN ; STRAINS ; LEGUMES ; NODULES
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/318666
作者单位	1.Maharana Pratap Univ Agr & Technol, Rajasthan Coll Agr, Dept Mol Biol & Biotechnol, Udaipur, Rajasthan, India; 2.Maharana Pratap Univ Agr & Technol, Rajasthan Coll Agr, Dept Soil Sci & Agr Chem, Udaipur, Rajasthan, India; 3.Manipal Univ Jaipur, Dept Biosci, Jaipur, Rajasthan, India; 4.CCS Haryana Agr Univ, Dept Microbiol, All India Network Project Soil Biodivers Bioferti, Hisar 125004, Haryana, India; 5.ICAR Indian Inst Soil Sci, All India Network Project Soil Biodivers Bioferti, Bhopal, India
推荐引用方式 GB/T 7714	Jain, Devendra,Kumari, Anisha,Saheewala, Heena,et al. Biochemical, functional and molecular characterization of pigeon pea rhizobia isolated from semi-arid regions of India[J],2020,202(7):1809-1816.
APA	Jain, Devendra.,Kumari, Anisha.,Saheewala, Heena.,Sanadhya, Suman.,Maheshwari, Deepali.,...&Mohanty, S. R..(2020).Biochemical, functional and molecular characterization of pigeon pea rhizobia isolated from semi-arid regions of India.ARCHIVES OF MICROBIOLOGY,202(7),1809-1816.
MLA	Jain, Devendra,et al."Biochemical, functional and molecular characterization of pigeon pea rhizobia isolated from semi-arid regions of India".ARCHIVES OF MICROBIOLOGY 202.7(2020):1809-1816.