干旱区生态环境知识资源中心(Arid): Identification of specific DNA markers for two isolated populations of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)

Arid

DOI	10.4001/003.028.0095
	Identification of specific DNA markers for two isolated populations of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)
	Jamal, Z. A.1; El-Deeb, N.1,2; Ghareeb, A.3; Ibrahim, H. A. M.4
通讯作者	Ghareeb, A.
来源期刊	AFRICAN ENTOMOLOGY
ISSN	1021-3589
EISSN	1026-4914
出版年	2020
卷号	28 期号:1 页码:95-105
英文摘要	Genomic DNA from two isolated gregarious locust populations in Africa had been amplified by using short decamer primers of random sequences. To identify markers that may distinguish between two major locust populations, northern Schistocerca gregaria gregaria, and southern Schistocerca gregaria flaviventris, 20 operon kit (A) primers from both populations were investigated with genomic DNA. Four primers generated low molecular weight polymorphic patterns. The primer (A2) showed a particular DNA amplified band (similar to 600 bp) from the southern S. g. flaviventris that was not expressed in the northern S. g. gregaria. For further examination, southern blot hybridisation indicated that this sequence is extremely repeated in the genome of the S. g. flaviventris population but not to the northern population. Therefore, this sequence may be highly specific to the southern locusts. Also, the southern population may constitute an evolutionary dichotomy that occurred on the African continent and can separate the populations of the two locusts into two distinct species.
英文关键词	locust populations Schistocerca gregaria gregaria Schistocerca gregaria flaviventris geographical isolation RAPD-PCR Southern blot hybridisation
类型	Article
语种	英语
国家	Saudi Arabia ; Egypt
收录类别	SCI-E
WOS记录号	WOS:000538533200011
WOS关键词	MOLECULAR MARKERS ; GENETIC DIVERSITY ; RAPD ; INSECT ; SOUTHERN ; MOTH
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/318518
作者单位	1.Taibuh Univ, Fac Sci Yanbu, Biol Dept, Al Sharm, Saudi Arabia; 2.Ain Shams Univ, Fac Women Arts Sci & Educ, Zool Dept, Cairo, Egypt; 3.Cairo Univ, Fac Sci, Microbiol Dept, Cairo, Egypt; 4.Ain Shams Univ, Fac Sci, Entomol Dept, Cairo, Egypt
推荐引用方式 GB/T 7714	Jamal, Z. A.,El-Deeb, N.,Ghareeb, A.,et al. Identification of specific DNA markers for two isolated populations of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)[J],2020,28(1):95-105.
APA	Jamal, Z. A.,El-Deeb, N.,Ghareeb, A.,&Ibrahim, H. A. M..(2020).Identification of specific DNA markers for two isolated populations of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae).AFRICAN ENTOMOLOGY,28(1),95-105.
MLA	Jamal, Z. A.,et al."Identification of specific DNA markers for two isolated populations of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)".AFRICAN ENTOMOLOGY 28.1(2020):95-105.