干旱区生态环境知识资源中心(Arid): 干旱胁迫下蒙古冰草的蛋白质组学分析

Arid

	干旱胁迫下蒙古冰草的蛋白质组学分析
其他题名	Proteomics Analyze of Agropyron mongolicum Keng in Drought
	王月英; 黄广学; 孟利前; 肖海峻
来源期刊	沈阳农业大学学报
ISSN	1000-1700
出版年	2020
卷号	51 期号:1 页码:105-110
中文摘要	蒙古冰草(Agropyron mongolicum Keng)是禾本科冰草属多年生草本植物,对干旱、寒冷及风沙具有很强的抵抗力,是干旱草原和荒漠地带的重要牧草。iTRAQ(isobaric tags for relative and absolute quantification)技术即同位素相对标记与绝对定量技术,是近年来最新开发的一种新的蛋白质组学定量研究技术,具有分离能力强、定量结果可靠、灵敏度高、可检测出低丰度蛋白等特点。以蒙古冰草为材料进行干旱处理12d后复水处理,分别在处理0,4,8,12,16d取样,利用iTRAQ技术分析蒙古冰草叶片在干旱胁迫及复水处理下蛋白质组学的变化,并进行生物信息学分析,研究结果表明:在干旱情况下持续上调的蛋白15个,持续下调的蛋白6个,在干旱复水处理中发现有1个基因(Q40001)是在干旱处理中处于下调状态,但是在复水处理后处于上调状态,通过查询蛋白的注释信息发现,该蛋白是一种镁离子螯合酶,其含量的变化与叶绿素的变化趋势相符。
英文摘要	Agropyron mongolicum Keng is a kind of perenial herb in Gramineae, It is an important pasture in arid grasslands and desert areas for its strong resistance to drought, cold and wind.iTRAQ, also known as isobaric tags for relative and absolute quantification, which is a newly developed technique for quantitative study in proteomics, has an advantage of strong separation ability, reliable in result, high sensitivity and can detect low abundance protein.In this experiment, The Agropyron mongolicum Keng was disposed in drought for 4, 8 and 12 days and rehydration for 4 days after 12 days drought treatment, and then their leaves proteomics variation and bioinformatics in drought and rehydration was analyzed by iTRAQ technique.The result revealed that 15 up-regulated proteins and 6 down-regulated proteins in dry condition,one gene from the rehydration sample of Agropyron called Q40001 was decrease in down -regulated status in drought, while it was up -regulated status after rehydration.It is confirmed that this protein is a kind of Magnesium ion chelase, which variation is corresponded with the variation of chlorophyll content.
中文关键词	蒙古冰草 ; 蛋白组学 ; ITRAQ技术
英文关键词	Agropyron mongolicum Keng proteomics TRAQ technique
语种	中文
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:6678735
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/318422
作者单位	北京农业职业学院, 北京 102442, 中国
推荐引用方式 GB/T 7714	王月英,黄广学,孟利前,等. 干旱胁迫下蒙古冰草的蛋白质组学分析[J],2020,51(1):105-110.
APA	王月英,黄广学,孟利前,&肖海峻.(2020).干旱胁迫下蒙古冰草的蛋白质组学分析.沈阳农业大学学报,51(1),105-110.
MLA	王月英,et al."干旱胁迫下蒙古冰草的蛋白质组学分析".沈阳农业大学学报 51.1(2020):105-110.