干旱区生态环境知识资源中心(Arid): 荒漠肉苁蓉多糖提取及纯化工艺优化

Arid

	荒漠肉苁蓉多糖提取及纯化工艺优化
其他题名	Process optimization for extraction and purification of polysaccharides from Cistanche deserticola
	肖星辉 1; 高海霞 2; 李鸿辉 2; 姬晓慧 2; 崔肖南 2; 李桂芳 2; 李予霞 2
来源期刊	中国中药杂志
ISSN	1001-5302
出版年	2019
卷号	44 期号:3 页码:475-481
中文摘要	该研究以新疆荒漠肉苁蓉为实验材料,通过单因素、正交实验探究水浸提法提取荒漠肉苁蓉多糖的最优工艺;通过 Sevag法和酶法及两者联用探究其对蛋白去除和对多糖保留的效果,确定荒漠肉苁蓉多糖最适去蛋白法;通过AB-8大孔树脂法和活性炭法探究脱色和纯化方法,确定肉苁蓉多糖纯化的最佳工艺.结果表明,水浸提法对荒漠肉苁蓉多糖提取的最佳工艺为提取温度75 ℃,提取时间165 min,料液比1∶ 55,在此工艺下,荒漠肉苁蓉多糖提取率为18.40%;最适去蛋白工艺为温度50 ℃,时间为2 h,木瓜蛋白酶用量为0.2%,在此脱蛋白体系下,脱蛋白率可达31.40%,多糖的保留率可达96.00%;在荒漠肉苁蓉多糖脱色方面,活性炭脱色率为80.37%,多糖的回收率为77.05%,AB-8大孔树脂脱色率为86.43%,多糖的回收率为91.93%,表明宜用大孔树脂进行脱色; AB-8大孔树脂纯化工艺为:上样浓度取4 g·L~(-1),洗脱液的浓度为60%乙醇,流速1 mL ·min~(-1),多糖粗提取液的纯度从67.70%升高至84.80%,纯化效果明显.
英文摘要	In this study,taking Cistanche deserticola in Xinjiang as the experimental material,the optimal process for extracting polysaccharides from C. deserticola with water extraction was studied by using single factor and orthogonal experiment. Its effects on protein removal and polysaccharides retaining were investigated by using Sevag,enzymatic method or combination of these two methods, so as to determine the optimal method for protein removal from polysaccharides of C. deserticola; the decolorization and purification methods such as macroporous resin of AB-8 and activated Carbon were used to determine the optimal process. The results showed that the extraction rate of polysaccharides from C. deserticola was 18.40% during the optimal process of the water extraction as follows: extraction temperature 75 ℃,extraction time 165 min and solid-liquid ratio 1∶ 55. The protein removal rate can reach 31.40% and polysaccharide retention rate can reach 96.00% under the optimal protein removal process: temperature 50 ℃,time 2 h,and papain dosage 0.2%. The decolorization rate of activated Carbon and macroporous resin called AB - 8 was 80.37% and 86.43%,and the recovery rate of polysaccharides was 77.05% and 91.93%,respectively,suggesting that macroporous resin was more suitable for decoloration. Macroporous resin named AB - 8 increased the purity of the polysaccharide crude extract from 67.70% to 84.80% under the following conditions: concentration of the sample 4 g·L~(-1),concentration of the eluent 60% ethanol,and the flow rate 1 mL·min~(-1), showing significant purification effect.
中文关键词	荒漠肉苁蓉 ; 多糖 ; 脱蛋白 ; 纯化 ; AB-8大孔树脂
英文关键词	Cistanche deserticola polysaccharides protein removal purification macroporous resin AB-8
语种	中文
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:6480128
来源机构	石河子大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/316167
作者单位	1.石河子大学生命科学学院;;中国科学技术大学生命科学学院, ;;, 石河子;;合肥, 新疆;;安徽 832000;;230000, 中国; 2.石河子大学生命科学学院, 石河子, 新疆 832000, 中国
推荐引用方式 GB/T 7714	肖星辉,高海霞,李鸿辉,等. 荒漠肉苁蓉多糖提取及纯化工艺优化[J]. 石河子大学,2019,44(3):475-481.
APA	肖星辉.,高海霞.,李鸿辉.,姬晓慧.,崔肖南.,...&李予霞.(2019).荒漠肉苁蓉多糖提取及纯化工艺优化.中国中药杂志,44(3),475-481.
MLA	肖星辉,et al."荒漠肉苁蓉多糖提取及纯化工艺优化".中国中药杂志 44.3(2019):475-481.