干旱区生态环境知识资源中心(Arid): 孕期非那雄胺暴露致子代雄性小鼠生殖器官发育异常

Arid

	孕期非那雄胺暴露致子代雄性小鼠生殖器官发育异常
其他题名	Abnormal development of genital organs in offspring male mice exposed to finasteride during pregnancy
	范霞; 李飞; 黄璜
来源期刊	中国病理生理杂志
ISSN	1000-4718
出版年	2020
卷号	36 期号:2 页码:323-328
中文摘要	目的:探讨孕期非那雄胺暴露对子代雄性小鼠生殖器官发育的影响。方法:CD-1小鼠在受孕后0~17d给予非那雄胺处理,通过宏观观察、解剖分析与组织形态学染色观察子代雄性小鼠生殖器官的发育情况;通过免疫荧光染色分析子代雄性小鼠精子发生情况。结果:宏观观察结果显示,孕期非那雄胺暴露可导致子代雄性小鼠外生殖器官畸形,表现为阴囊未完全融合及阴茎畸形;此外,还观察到小鼠肛门与生殖器的距离显著缩短(P <0.01)。解剖分析结果显示,孕期非那雄胺暴露可导致子代雄性小鼠睾丸不同程度的不完全下降及长度显著缩短(P <0.01)。组织形态学结果显示,各阶段阴茎长度均显著缩短(P <0.01);睾丸生精小管密度和生精小管管腔成熟精子数均显著降低(P <0.01),生精小管管腔和睾丸间质间隙均显著增大(P <0.01)。免疫荧光染色结果显示,睾丸中支持细胞、睾丸间质细胞和精原细胞的密度均显著降低(P <0.01);生精小管细胞的caspase-3荧光强度显著增加(P <0.01),Ki67与沙漠刺猬因子(desert hedgehog,Dhh)荧光强度均显著降低(P <0.01)。结论:孕期非那雄胺暴露可导致子代小鼠生殖器官发育异常并影响精子发生。
英文摘要	AIM:To investigate the effects of finasteride exposure during pregnancy on the development of reproductive organs in offspring male mice.METHODS:CD-1 mice were treated with finasteride from 0 to 17 d after conception.The development of reproductive organs in the offspring male mice were observed by macroscopy,anatomical analysis and histological staining.The spermatogenesis was analyzed by immunofluorescence staining.RESULTS:Macroscopic observation showed that fenasteride exposure during pregnancy resulted in external genital malformations in the offspring male mice,which were manifested as incomplete scrotal fusion and penile malformation.In addition,the anogenital distance was significantly shortened (P <0.01).Histological staining showed that the length of penis at each stage was significantly shortened (P <0.01),the density of seminiferous tubules in testis and mature spermatozoa in seminiferous tubules were significantly decreased (P <0.01),and the luminal space size of seminiferous tubules and interstitial space size of testises were significantly increased (P <0.01).Immunofluorescence staining showed that the densities of Sertoli cells,Leydig cells and spermatogonium in testis were significantly reduced (P <0.01),and the fluorescence intensity of caspase-3 in spermatogenic tubule cells was increased significantly (P <0.01).However,the fluorescence intensity of Ki67 and desert hedgehog (Dhh) was decreased significantly (P <0.01).CONCLUSION:Finasteride exposure during pregnancy results in abnormal development of reproductive organs in offspring male mice and affects spermatogenesis.
中文关键词	非那雄胺 ; 孕期暴露 ; 性发育障碍 ; 生殖器官 ; 精子发生
英文关键词	Finasteride Prenatal exposure Sexual developmental disorder Reproductive organs Spermatogenesis
语种	中文
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:6665495
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/316109
作者单位	贵州省人民医院儿外科, 贵阳, 贵州 550001, 中国
推荐引用方式 GB/T 7714	范霞,李飞,黄璜. 孕期非那雄胺暴露致子代雄性小鼠生殖器官发育异常[J],2020,36(2):323-328.
APA	范霞,李飞,&黄璜.(2020).孕期非那雄胺暴露致子代雄性小鼠生殖器官发育异常.中国病理生理杂志,36(2),323-328.
MLA	范霞,et al."孕期非那雄胺暴露致子代雄性小鼠生殖器官发育异常".中国病理生理杂志 36.2(2020):323-328.