干旱区生态环境知识资源中心(Arid): 蛋白基因表达谱分析

Arid

	蛋白基因表达谱分析
其他题名	Antennal transcriptome analysis and expression profiling of chemosensory protein genes in Oedaleus asiaticus (Orthopera: Acrididae)
	周渊涛 ; 李玲 ; 庞保平 ; 单艳敏 ; 张卓然
来源期刊	昆虫学报
ISSN	0454-6296
出版年	2019
卷号	62 期号:1 页码:21-32
中文摘要	【目的】化学感受蛋白(chemosensory proteins, CSPs)是一类小分子的可溶性蛋白,在昆虫中发挥多种作用.本研究旨在组装中国北方主要草原害虫之一--亚洲小车蝗Oedaleus asiaticus的触角转录组,鉴定出化学感受蛋白基因以及分析其在成虫不同组织中的表达水平.【方法】利用RNA-Seq对亚洲小车蝗成虫触角进行转录组测序和组装;通过筛选转录组数据库、克隆及测序,鉴定出化学感受蛋白基因;应用qPCR分析CSP基因在成虫不同组织(触角、去除触角和口器的头、上唇、去掉下唇须的下唇、下唇须、下颚须、胸部、跗节、翅和腹部)中的表达模式.【结果】成功构建了亚洲小车蝗成虫触角转录组,共获得61 629个unigenes,平均长度为733 nt,总长度和N50分别为45 175 449和1 130 nt.其中26 064个unigenes(42.29%)注释到6个数据库(NR, NT, Swiss-Prot, KEGG, COG和GO).通过Blast验证、克隆和测序鉴定出17个CSP基因;BlastP和系统发育分析表明亚洲小车蝗CSPs(OasiCSPs)与东亚飞蝗Locusta migratoria CSPs(LmigCSPs)和沙漠蝗Schistocerca gregaria CSPs(SgreCSPs)关系最密切.qPCR分析表明,8个CSP基因在成虫不同组织中的表达水平存在显着差异,特别是,OasiCSP8在下唇须和下颚须中高表达,而OasiCSP11和OasiCSP13在触角中表达量最高.OasiCSP15在化学感受器官(触角、上唇、去掉下唇须的下唇、下唇须、下颚须和跗节)中的表达量远高于非化学感受器官(去掉触角和口器的头部、胸部、翅和腹部)中的表达量,而OasiCSP12在几乎所有测定的组织中具有相似的表达分布.【结论】OasiCSPs在亚洲小车蝗化学感受和发育过程中可能起着多种作用,这些结果为进一步研究这些化学感受蛋白在亚洲小车蝗中的功能奠定了基础.
英文摘要	【Aim】 Chemosensory proteins (CSPs) are soluble carrier proteins that may play multiple roles in insects. The objective of this study is to assembly the antennal transcriptomes of Oedaleus asiaticus, one of the most important grasshopper pests in northern China, to identify the chemosensory protein genes, and to analyze their tissue expression profiles. 【Methods】 The antennal transcriptomes of the O. asiaticus adults were sequenced and assembled by RNA-Seq. The CSP genes were identified by screening the transcriptome data, and their expression levels in different adult tissues (antenna, head without antennae and mouthparts, labrum, labium without labial palps, labial palp, maxillary palp, thorax, tarsus, wing, and abdomen) were analyzed by qPCR. 【Results】 The antennal transcriptomes of the O. asiaticus adults were successfully assembled. A total of 61 629 unigenes with a mean length of 733 nt were obtained, and the total length and N50 were 45 175 449 nt and 1 130 nt, respectively. Among them, 26 064 unigenes (42.29%) were annotated to six databases (NR, NT, Swiss-Prot, KEGG, COG and GO). A total of 17 putative CSP genes were identified by blasting, cDNA cloning and sequencing. BlastP best hit results and phylogenetic analysis both showed that the putative OasiCSPs were most closely related to LmigCSPs from Locusta migratoria and SgreCSPs from Schistocerca gregaria. qPCR analysis showed that there were significant differences in the expression levels of eight different CSP genes in different adult tissues. Especially, OasiCSP8 was much highly expressed in the labial palps and maxillary palps, and OasiCSP11 and OasiCSP13 had the highest expression levels in the antenna. OasiCSP15 had much higher expression levels in chemosensory tissues (antenna, labrum, labium without labial palps, labial palp, maxillary palp, and tarsus) than in non-chemosensory tissues (head without antenna and mouthpart, thorax, wing, and abdomen). However, OasiCSP12 had similar expression distribution in nearly all the tested tissues.【Conclusion】 The results suggest that OasiCSPs might play multiple roles in the chemosensory process and development of O. asiaticus. Our findings provide a foundation for the further functional studies of these CSPs in O. asiaticus.
中文关键词	亚洲小车蝗 ; 触角 ; 转录组 ; 化学感受蛋白 ; 表达谱
英文关键词	Oedaleus asiaticus antenna transcriptome chemosensory protein expression profile
语种	英语
收录类别	CSCD
WOS类目	ENTOMOLOGY
WOS研究方向	Entomology
CSCD记录号	CSCD:6418715
来源机构	内蒙古农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/316019
作者单位	1.Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010020, China; 2.Inner Mongolia Grassland Station, Hohhot, Inner Mongolia 010020, China
推荐引用方式 GB/T 7714	周渊涛,李玲,庞保平,等. 蛋白基因表达谱分析[J]. 内蒙古农业大学,2019,62(1):21-32.
APA	周渊涛,李玲,庞保平,单艳敏,&张卓然.(2019).蛋白基因表达谱分析.昆虫学报,62(1),21-32.
MLA	周渊涛,et al."蛋白基因表达谱分析".昆虫学报 62.1(2019):21-32.