干旱区生态环境知识资源中心(Arid): 基于高通量测序技术分析2种菌草根际土壤细菌群落多样性

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	基于高通量测序技术分析2种菌草根际土壤细菌群落多样性
其他题名	Rhizosphere Soil Bacterial Community of Juncao by High-throughput Sequencing Techniques
	叶文雨 1; 廖海萍 2; 许钰滢 2; 谢序泽 2; 倪明月 3; 胡红莉 3; 余文英 2; 鲁国东 4
来源期刊	热带作物学报
ISSN	1000-2561
出版年	2019
卷号	40 期号:9 页码:1783-1788
中文摘要	为详细了解福州菌草基地巨菌草和绿洲一号2种菌草的根际土壤细菌群落组成与结构,本研究应用高通量测序技术,分析了巨菌草和绿洲一号根际土壤细菌群落结构及多样性。结果表明,菌草根际土检测到细菌类群主要的优势菌门为酸杆菌门(Acidobacteria)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadetes)、硝化螺旋门(Nitrospirae)、疣微菌门(Verrucomicrobia)、浮霉菌门(Planctomycetes);主要的优势菌纲为gamma-变形菌纲(Gammaproteobacteria)、alpha-变形菌纲(Alphaproteobacteria)、芽孢杆菌纲(Bacilli)、放线菌纲(Actinobacteria)、酸杆菌纲(Acidobacteria-6)、beta-变形菌纲(Betaproteobacteria)、酸杆菌纲(Acidobacteriia)、纤线杆菌纲(Ktedonobacteria)、delta-变形菌纲(Deltaproteobacteria);主要的优势属为葡萄球菌属(Staphylococcus)、糖多孢菌属(Saccharopolyspora)、类芽孢杆菌属(Paenibacillus)、沙雷氏菌属(Serratia)、链球菌属(Streptococcus)、红游动菌属(Rhodoplanes)、不动细菌属(Acinetobacter)、假单胞菌属(Pseudomonas)。绿洲一号、巨菌草及其对照根际土壤细菌Shannon-Wiener指数分别为9.05、8.733、7.61;巨菌草、绿洲一号及其对照根际土壤细菌Simpson多样性指数分别为0.01、0.014、0.037;巨菌草、绿洲一号及其对照根际土壤细菌Chao1指数分别为3145.320、3647.482、2329.344。Alpha多样性指数分析得出,在3个土壤样品中,绿洲一号根际土Shannon指数最大,对照土壤Shannon指数最小,对照土壤Simpson指数最大,绿洲一号Simpson指数最小,说明菌草根际土壤细菌群落多样性高于非根际土土壤细菌群落多样性,绿洲一号根际土土壤细菌群落多样性略高于巨菌草根际土壤细菌群落多样性,这些结果为进一步研究土壤微生物提供了理论基础。
英文摘要	Diversity analyses were investigated by the high-throughput sequencing to study the composition and diversity of bacterial communities in the rhizosphere soil of Pennisetum giganteum and Arundo donax cv. Lvzhou No. 1. Results showed that the dominant phyla of all samples were Acidobacteria, Proteobacteria, Actinobacteria, Firmicutes, Chloroflexi, Gemmatimonadetes, Nitrospirae, Verrucomicrobia, Planctomycetes. At the class level, nine dominant species were found in all samples, such as Gammaproteobacteria, Alphaproteobacteria, Bacilli, Actinobacteria, Acidobacteria- 6, Betaproteobacteria, Acidobacteriia, Ktedonobacteria, Deltaproteobacteria. At the genus level, dominant species were found in all samples, such as Staphylococcus, Saccharopolyspora, Paenibacillus, Serratia, Streptococcus, Rhodoplanes, Acinetobacter, Pseudomonas. The Shannon-Wiener from the samples was 9.05, 8.733, 7.61, respectively. The Simpson index from the samples was 0.01, 0.014, 0.037, respectively. The Chao1 index from the samples was 3145.320, 3647.482, 2329.344, respectively. The result of Alpha diversity analysis showed that the Shannon index, Simpson index and Chao1 index of Juncao increased. The diversity index analysis showed that in the soil samples, the lowest bacterial diversity was in the experimental control soil and the highest bacterial diversity was in the A. donax cv. Lvzhou No. 1 rhizosphere soil. The bacterial diversity in the A. donax cv. Lvzhou No. 1 rhizosphere soil was slightly higher than that in the Pennisetum giganteum rhizosphere soil.
中文关键词	菌草 ; 根际土壤 ; 细菌群落多样性 ; 高通量测序
英文关键词	Juncao rhizosphere soil diversity of bacterial communities high-throughput sequencing
语种	中文
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6581403
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315962
作者单位	1.福建农林大学;;福建农林大学生命科学学院, 国家菌草工程技术研究中心;;, 福州;;福州, 福建;;福建 350002;;350002, 中国; 2.福建农林大学生命科学学院, 福州, 福建 350002, 中国; 3.福建农林大学植物保护学院, 福州, 福建 350002, 中国; 4.福建农林大学;;福建农林大学植物保护学院, 国家菌草工程技术研究中心;;, 福州;;福州, 福建;;福建 350002;;350002, 中国
推荐引用方式 GB/T 7714	叶文雨,廖海萍,许钰滢,等. 基于高通量测序技术分析2种菌草根际土壤细菌群落多样性[J],2019,40(9):1783-1788.
APA	叶文雨.,廖海萍.,许钰滢.,谢序泽.,倪明月.,...&鲁国东.(2019).基于高通量测序技术分析2种菌草根际土壤细菌群落多样性.热带作物学报,40(9),1783-1788.
MLA	叶文雨,et al."基于高通量测序技术分析2种菌草根际土壤细菌群落多样性".热带作物学报 40.9(2019):1783-1788.